2011-2021 | 全自动Digital Western Blot十年助力Merck研发与生产

自2011年，ProteinSimple上市第一代全自动Digital Western Blot系统Simon以来，十年间，我们不断倾听客户的声音，不断创新优化，推出Sally Sue、Wes、Peggy Sue、NanoPro1000、Jess和Abby系统。

在生物制品蓬勃发展的十年中，无论是“百抗”、双抗、ADC、PROTAC、AbTACs（双特异性抗体+PROTACs）、疫苗（埃博拉疫苗、肺炎疫苗、人类巨细胞病毒疫苗、四价艰难俊菌疫苗）、细胞和基因治疗领域，ProteinSimple全自动Digital Western Blot都躬身入局。

Merck作为世界制药企业的领先者，十年间，ProteinSimple与Merck深度合作，助力药物研发（药效动力学、靶点验证等等）、疫苗研发和生产。包括，全球首个埃博拉疫苗ERVEBO®和15价肺炎链球菌疫苗上市。仅在Merck美国宾夕法尼亚州West Point，一个Site，就有26台全自动Digital Western Blot系统运行。全球范围内Merck多个Site和部门，也都遍地开花。

ProteinSimple全自动Digital Western Blot系统，以其高度自动化、高质量数据全面助力生物制品行业上游和下游相关工作。

在此，分享Merck公司利用全自动Digital WB系统，十年间，发表过的18篇文章。

Establishing and Validating Cellular Functional Target Engagement Assay for Selective IRAK4 Inhibitor Discovery

全自动Digital Western Blot检测系统：Peggy Sue

药物研发：药效动力学

Site：Department of Quantitative Bioscience&Department of Discovery Chemistry, Merck & Co., Inc., Boston, MA, USA

Development of a novel capillary electrophoresis-based approach for detection of anti-drug antibody responses

全自动Digital Western Blot检测系统：Peggy Sue

药物发现：临床前ADA（ Anti-Drug Antibodies） 分析鉴定

Site：PPDM&Biologics, Merck & Co., Inc., 213 E Grand Ave, South San Francisco, CA 94080, USA 

Characterization of gH/gL/pUL128-131 pentameric complex, gH/gL/gO trimeric complex, gB and gM/gN glycoproteins in a human cytomegalovirus using automated capillary western blots

全自动Digital Western Blot检测系统：Wes

人类巨细胞病毒疫苗：定量人巨细胞病毒疫苗多种抗原水平

Site: Vaccine Analytical Research Development and Vaccine Process Development Merck & Co., Inc., Kenilworth, NJ, USA

Characterization of rVSVΔG-ZEBOV-GP glycoproteins using automated capillary western blotting

全自动Digital Western Blot检测系统：Wes

埃博拉疫苗：全球首个上市预防埃博拉病毒病的商品化疫苗ERVEBO®

图片来源于Merck官网

Site: Vaccine Analytical Research Development and Vaccine Process Development and Commercialization, Merck & Co., Inc., Kenilworth, NJ, USA

A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice

全自动Digital Western Blot检测系统：Peggy Sue

药物靶点验证

Site：Translational Innovation Platform Oncology, Merck KGaA, Darmstadt, Germany等

A capillary electrophoresis based approach for the identification of anti-drug antibodies against camelid VHH biologics (Nanobodies®)

全自动Digital Western Blot检测系统：Wes

药物发现：针对Nanobody的ADA鉴定

Site:  PPDM ADME Biologics, PPDM Bioanalytics, Immunology and IMRs, MRL, Merck & Co., Inc., 213 E. Grand Avenue, South San Francisco, CA 94080, USA

Development of an ADP-ribosylation assay for residual toxicity in C.difficile binary toxin CDTa using automated capillary western blot

全自动Digital Western Blot检测系统：Peggy Sue

四价艰难俊菌疫苗：ADP 核糖基化活性

Site: Vaccine Research Analytical Development, Merck & Co., Inc., Kenilworth, NJ, USA

Efficacy and Pharmacodynamic Modeling of the BTK Inhibitor Evobrutinib in Autoimmune Disease Models

全自动Digital Western Blot检测系统：Wes

药物发现：BTK抑制剂Evobrutinib

Site: Translational Innovation Platform Immunology, Merck KGaA, Darmstadt 64293,

Germany; Immunology, Merck Serono, 1201 Geneva, Switzerland等

2017

Quantitation of CRM197 using imaged capillary isoelectric focusing with fluorescence detection and capillary Western

全柱成像毛细管等电聚焦电泳系统：Maurice

全自动Digital Western Blot检测系统：Wes

疫苗生产杂质鉴定：白喉毒素

Site：Biologics and Vaccines 8 Merck Sharp & Dohme, West Point, PA 19486, USA

An integrin antagonist (MK‐0429) decreases proteinuria and renal fibrosis in the ZSF1 rat diabetic nephropathy model

全自动Digital Western Blot检测系统：Sally Sue

药物研发：肾病药物

Site：Department of Pharmacology, Department of Cardiometabolic Diseases&Department of Medicinal Chemistry, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, New Jersey 07033

Neutralization of Diverse Human Cytomegalovirus Strains Conferred by Antibodies Targets Viral gH/gL/pUL128-131 Pentameric Complex

全自动Digital Western Blot检测系统：Simon

人类巨细胞病毒疫苗：抗体检测

Site：Merck Research Laboratories, Merck and Co., Inc., Kenilworth, New Jersey, USA等

Ability of Bruton’s Tyrosine Kinase Inhibitors to Sequester Y551 and Prevent Phosphorylation Determines Potency for Inhibition of Fc Receptor but not B-Cell Receptor Signaling

全自动Digital Western Blot检测系统：Wes

免疫学：Btk（布鲁顿酪氨酸激酶）磷酸化检测

Site：TIP Immunology, Merck, Darmstadt, Germany等

2016

Applications of an Automated and Quantitative CE-Based Size and Charge Western Blot for Therapeutic Proteins and Vaccines

全自动Digital Western Blot检测系统：Sally, Sally Sue, Peggy, Peggy Sue, Wes

四价艰难梭菌疫苗: 筛选细胞克隆株、免疫学鉴别和稳定性研究

Site: NA

2015

Automated capillary Western dot blot method for the identity 4 of a 15-valent pneumococcal conjugate vaccine

全自动Digital Western Blot检测系统：Peggy Sue

15价肺炎链球菌疫苗免疫学鉴别

图片来源于Merck官网

Site: Vaccine Analytical Development, Merck Research Laboratories, West Point, PA 19486, USA

Soluble Human Cytomegalovirus gH/gL/pUL128-131 Pentameric Complex, but not gH/gL, Inhibits Viral Entry to Epithelial Cells and Presents Dominant Native Neutralizing Epitopes

全自动Digital Western Blot检测系统：NanoPro

人类巨细胞病毒疫苗：gH/gL 和gH/gL/pUL128–131五聚体蛋白检测

Site: Vaccine Bioprocess Research & Development, Merck Research Laboratories, West Point, PA 19486, USA

2014

Residual bovine serum albumin (BSA) quantitation in vaccines using automated Capillary Western technology

型号：NA

疫苗牛血清白蛋白（BSA）杂质检测

Site：Vaccine Analytical Development, Merck Research Laboratories, West Point, PA 19486, USA

Effects of anacetrapibon plasma lipids, apolipoproteins and PCSK9 in healthy,lean rhesus macaques

全自动Digital Western Blot检测系统：Sally Sue

心血管药理学：低密度脂蛋白受体蛋白水平检测

Site: Merck Research Laboratories, Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA

2012

Qualitative and quantitative evaluation of SimonTM, a new CE-based automated Western blot system as applied to vaccine development

全自动Digital Western Blot检测系统：Simon

候选疫苗定量检测：CV ＜ 10%

Site: Vaccine Analytical Development, Merck Research Laboratories, West Point, PA, USA

下一个十年，我们依旧相伴！