论文分享 | 假体周围骨溶解治疗新策略

The BCA Protein Quantification Kit is used to determine the protein concentration in exosomes. M2-exo was mixed with USC-exo and subjected to ultrasonic membrane fusion using a SCIENTZ-IID Ultrasonic Homogenizer (Ningbo Scientz Biotechnology Co., Ltd, China.) for 3 min.This was followed by repeated co-extrusion with the polycarbonate membrane of the liposome extruder (100 nm, 50 nm), forming the f-exo.

BCA蛋白质定量试剂盒用于测定外泌体中的蛋白质浓度。将M2-exo与USC-exo混合后，使用SCIENTZ-IID型超声波破碎仪（宁波新芝生物科技股份有限公司，中国）进行3分钟超声膜融合；随后通过脂质体挤出机的聚碳酸酯膜（100 nm、50 nm孔径）反复共挤出，最终制备得到f-exo。

SCIENTZ- IID 超声破碎仪