RTCA实时无标记细胞分析技术应用文献更新

实时无标记细胞分析技术(RTCA, Real Time Cell Analysis)是艾森生物 (ACEA Biosciences) 全球独有的专利核心技术。该技术采用特殊工艺，将微电极列阵整合在细胞培养板的每个细胞生长孔底部，用以构建实时、动态、定量跟踪细胞形态和增殖分化改变的细胞阻抗检测传感系统。目前，应用RTCA技术所发表的文献已近500篇，其中影响因子在10以上的有14篇，影响因子在5以上的有140篇，以下是应用RTCA技术最新更新的文献列表及相关链接：

RTCA应用领域（Research Areas）细胞屏障 Cell Barrier Function 细胞粘附及伸展 Cell Adhesion/Spreading 细胞增殖 Cell Proliferation 细胞浸润及迁移 Cell Invasion and Migration免疫学 Immunology 微生物、病毒学及寄生虫病Microbiology, Virology and Parasitology细胞毒性及死亡 Cytotoxicity and Cell Death 细胞心脏病学研究 Cellular Cardiology Research

细胞屏障 （Cell Barrier Function）Kustermann S., et al. A Real-time Impedance-Based Screening Assay for Drug-Induced Vascular Leakage.Toxicol Sci. 2014 Jan 11.

Kida T., et al. Stimulation of G protein-coupled bile acid receptor enhances vascular endothelial barrier function via activation of protein kinase A and Rac1. J Pharmacol Exp Ther. 2014 Jan;348(1):125-30.

Faurobert E., et al. CCM1-ICAP-1 complex controls β1 integrin-dependent endothelial contractility and fibronectin remodeling. J Cell Biol. 2013 Aug 5;202(3):545-61.Gan AM ., et al. Inflammatory effects of resistin on human smooth muscle cells: up-regulation of fractalkine and its receptor, CX3CR1 expression by TLR4 and Gi-protein pathways. Cell Tissue Res. 2013 Jan;351(1):161-74. Mohammed M. Shareef., et al. Interaction of HIF-1α and Notch3 Is Required for the Expression of Carbonic Anhydrase 9 in Breast Carcinoma Cells. Genes Cancer March 21, 2013

Chalubinski M., et al. The effect of 7-ketocholesterol and 25-hydroxycholesterol on the integrity of the human aortic endothelial and intestinal epithelial barriers. Inflamm Res. 2013 Sep 28.Kürti L., et al. Retinoic acid and hydrocortisone strengthen the barrier function of human RPMI 2650 cells, a model for nasal epithelial permeability. Cytotechnology. 2013 May;65(3):395-406.

细胞粘附及伸展（Cell Adhesion/Spreading）Faurobert E., et al. CCM1-ICAP-1 complex controls β1 integrin-dependent endothelial contractility and fibronectin remodeling. J Cell Biol. 2013 Aug 5;202(3):545-61.Braig S., et al. Indirubin derivative 6BIO suppresses metastasis. Cancer Res. 2013 Oct 1;73(19):6004-12.Matsumoto Y., et al. Trimeric Tn antigen on syndecan 1 produced by ppGalNAc-T13 enhances cancer metastasis via a complex formation with integrin α5β1 and matrix metalloproteinase 9. J Biol Chem. 2013 Aug 16;288(33):24264-76.Sabit I., et al. Binding of a sialic acid-recognizing lectin Siglec-9 modulates adhesion dynamics of cancer cells via calpain-mediated protein degradation. J Biol Chem. 2013 Dec 6;288(49):35417-27.Tibaldi L., et al. New blocking antibodies impede adhesion, migration and survival of ovarian cancer cells, highlighting MFGE8 as a potential therapeutic target of human ovarian carcinoma. PLoS One. 2013 Aug 16;8(8):e72708.

细胞增殖（Cell Proliferation）Weiler M., et al. mTOR target NDRG1 confers MGMT-dependent resistance to alkylating chemotherapy.Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):409-14.Iglesias-Gato D., et al. SOCS2 mediates the cross talk between androgen and growth hormone signaling in prostate cancer. Carcinogenesis. 2014 Jan;35(1):24-33. Shihadeh A., et al. Comparison of tobacco-containing and tobacco-free waterpipe products: effects on human alveolar cells. Nicotine Tob Res. 2014 Apr;16(4):496-9.Bartl J., et al. Enhancement of cell viability after treatment with polyunsaturated fatty acids. Neurosci Lett. 2014 Jan 24;559:56-60.Dreesen O., et al. Lamin B1 fluctuations have differential effects on cellular proliferation and senescence. J Cell Biol. 2013 Mar 4;200(5):605-17.Bang D., et al. GSK-3α promotes oncogenic KRAS function in pancreatic cancer via TAK1-TAB stabilization and regulation of noncanonical NF-κB. Cancer Discov. 2013 Jun;3(6):690-703.Kamphorst JJ., et al. Hypoxic and Ras-transformed cells support growth by scavenging unsaturated fatty acids from lysophospholipids.Proc Natl Acad Sci U S A. 2013 May 28;110(22):8882-7. Szabo C., et al. Tumor-derived hydrogen sulfide, produced by cystathionine-β-synthase, stimulates bioenergetics, cell proliferation, and angiogenesis in colon cancer. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12474-9.Hauer K., et al. DKK2 mediates osteolysis, invasiveness, and metastatic spread in Ewing sarcoma.Cancer Res. 2013 Jan 15;73(2):967-77.

Teng YC., et al. Histone demethylase RBP2 promotes lung tumorigenesis and cancer metastasis.Cancer Res.2013 Aug 1;73(15):4711-21.

Zaid TM., et al. Identification of FGFR4 as a potential therapeutic target for advanced-stage, high-grade serous ovarian cancer. Clin Cancer Res. 2013 Feb 15;19(4):809-20.

Su SC., et al. Autotaxin-lysophosphatidic acid signaling axis mediates tumorigenesis and development of acquired resistance to sunitinib in renal cell carcinoma. Clin Cancer Res. 2013 Dec 1;19(23):6461-72. Schrötter A., et al. FE65 regulates and interacts with the Bloom syndrome protein in dynamic nuclear spheres - potential relevance to Alzheimer's disease. J Cell Sci. 2013 Jun 1;126(Pt 11):2480-92. Niture SK., et al. Oncogene PKCε controls INrf2-Nrf2 interaction in normal and cancer cells through phosphorylation of INrf2. J Cell Sci. 2013 Dec 15;126(Pt 24):5657-69.Wang X., et al. Hypermethylation reduces expression of tumor-suppressor PLZF and regulates proliferation and apoptosis in non-small-cell lung cancers. FASEB J. 2013 Oct;27(10):4194-203.Bifulco K., et al. A urokinase receptor-derived peptide inhibiting VEGF-dependent directional migration and vascular sprouting. Mol Cancer Ther. 2013 Oct;12(10):1981-93.Schaale K., et al. Wnt6 is expressed in granulomatous lesions of Mycobacterium tuberculosis-infected mice and is involved in macrophage differentiation and proliferation. J Immunol. 2013 Nov 15;191(10):5182-95.

细胞浸润及迁移（Cell Invasion and Migration）Lahoti TS., et al. Aryl hydrocarbon receptor antagonism attenuates growth factor expression, proliferation, and migration in fibroblast-like synoviocytes from patients with rheumatoid arthritis. J Pharmacol Exp Ther. 2014 Feb;348(2):236-45.Prasadam I., et al. Osteocyte-induced angiogenesis via VEGF-MAPK-dependent pathways in endothelial cells. Mol Cell Biochem. 2014 Jan;386(1-2):15-25.Chugh PE., et al. Systemically circulating viral and tumor-derived microRNAs in KSHV-associated malignancies. PLoS Pathog. 2013;9(7):e1003484.Dunne PD., et al. AXL Is a Key Regulator of Inherent and Chemotherapy-Induced Invasion and Predicts a Poor Clinical Outcome in Early-Stage Colon Cancer. Clin Cancer Res. 2013 Dec 12.Sprenger A., et al. Consistency of the proteome in primary human keratinocytes with respect to gender, age, and skin localization. Mol Cell Proteomics. 2013 Sep;12(9):2509-21.Niland S., et al. Rhodocetin-αβ-induced neuropilin-1-cMet association triggers restructuring of matrix contacts in endothelial cells. Arterioscler Thromb Vasc Biol. 2013 Mar;33(3):544-54.

Wanschel A., et al. Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention. Arterioscler Thromb Vasc Biol. 2013 May;33(5):886-93.

Ngan E., et al. A complex containing LPP and α-actinin mediates TGFβ-induced migration and invasion of ErbB2-expressing breast cancer cells. J Cell Sci. 2013 May 1;126(Pt 9):1981-91. Giles KM., et al. Axl mediates acquired resistance of head and neck cancer cells to the epidermal growth factor receptor inhibitor erlotinib. Mol Cancer Ther. 2013 Nov;12(11):2541-58.Yuan L., et al. Growth factor receptor-Src-mediated suppression of GRK6 dysregulates CXCR4 signaling and promotes medulloblastoma migration. Mol Cancer. 2013 Mar 5;12:18.van Oosterwijk JG., et al. Src kinases in chondrosarcoma chemoresistance and migration: dasatinib sensitises to doxorubicin in TP53 mutant cells. Br J Cancer. 2013 Sep 3;109(5):1214-22.

免疫学（Immunology）Messaoudene M., et al. Mature cytotoxic CD56(bright)/CD16(+) natural killer cells can infiltrate lymph nodes adjacent to metastatic melanoma. Cancer Res. 2014 Jan 1;74(1):81-92.Orava EW., et al. Blocking the attachment of cancer cells in vivo with DNA aptamers displaying anti-adhesive properties against the carcinoembryonic antigen. Mol Oncol. 2013 Aug;7(4):799-811.Henle AM., et al. Enzymatic discovery of a HER-2/neu epitope that generates cross-reactive T cells. J Immunol. 2013 Jan 1;190(1):479-88.Sherwood CL., et al. Chronic arsenic exposure in nanomolar concentrations compromises wound response and intercellular signaling in airway epithelial cells. Toxicol Sci. 2013 Mar;132(1):222-34.Asif J. Iqbal, et al. A Real Time Chemotaxis Assay Unveils Unique Migratory Profiles amongst Different Primary Murine Macrophages. PLoS ONE. 2013; 3: e58744Guan N., et al. Label-free monitoring of T cell activation by the impedance-based xCELLigence system.Mol Biosyst. 2013 Apr 2;9(5):1035-43.Park KH., et al. Evaluation of NK cell function by flowcytometric measurement and impedance based assay using real-time cell electronic sensing system. Biomed Res Int. 2013;2013:210726.

微生物、病毒学及寄生虫病（Microbiology, Virology and Parasitology）Sharma BN., et al. Antiviral effects of artesunate on polyomavirus BK replication in primary human kidney cells. Antimicrob Agents Chemother. 2014 Jan;58(1):279-89.Ebersohn K., et al. An improved method for determining virucidal efficacy of a chemical disinfectant using an electrical impedance assay. J Virol Methods. 2014 Apr;199:25-8. You H., et al. Transcriptional responses of in vivo praziquantel exposure in schistosomes identifies a functional role for calcium signalling pathway member CamKII.PLoS Pathog. 2013 Mar;9(3):e1003254.Kim YR., et al. A bacterial RTX toxin causes programmed necrotic cell death through calcium-mediated mitochondrial dysfunction.J Infect Dis. 2013 May 1;207(9):1406-15.Varjak M., et al. Magnetic fractionation and proteomic dissection of cellular organelles occupied by the late replication complexes of Semliki Forest virus. J Virol. 2013 Sep;87(18):10295-312.Dazhi Jin., et al. Quantitative Detection of Vibrio cholera Toxin by Real-Time and Dynamic Cytotoxicity Monitoring. J Clin Microbiol. 2013 Dec;51(12):3968-74.Al-Ahmad A ., et al. Nature-inspired antimicrobial polymers--assessment of their potential for biomedical applications. PLoS One. 2013 Sep 9;8(9):e73812.Teng Z., et al. Real-time cell analysis - A new method for dynamic, quantitative measurement of infectious viruses and antiserum neutralizing activity. J Virol Methods. 2013 Jul 5;193(2):364-370.

细胞毒性及死亡（Cytotoxicity and Cell Death） Huang B., et al. Real-Time Cellular Analysis Coupled with a Specimen Enrichment Accurately Detects and Quantifies Clostridium difficile Toxins in Stool. J Clin Microbiol. 2014 Apr;52(4):1105-11.Hasan M., et al. Liposome encapsulation of curcumin: physico-chemical characterizations and effects on MCF7 cancer cell proliferation. Int J Pharm. 2014 Jan 30;461(1-2):519-28.Meindl C ., et al. Suitability of cell-based label-free detection for cytotoxicity screening of carbon nanotubes. Biomed Res Int. 2013;2013:564804.Hou XQ., et al. A novel assay for high-throughput screening of anti-Alzheimer's disease drugs to determine their efficacy by real-time monitoring of changes in PC12 cell proliferation. Int J Mol Med. 2014 Mar;33(3):543-9.Offer SM., et al. Phenotypic profiling of DPYD variations relevant to 5-fluorouracil sensitivity using real-time cellular analysis and in vitro measurement of enzyme activity. Cancer Res. 2013 Mar 15;73(6):1958-68.Zhou B., et al. A small-molecule blocking ribonucleotide reductase holoenzyme formation inhibits cancer cell growth and overcomes drug resistance. Cancer Res. 2013 Nov 1;73(21):6484-93.Rader J., et al. Dual CDK4/CDK6 Inhibition Induces Cell-Cycle Arrest and Senescence in Neuroblastoma.Clin Cancer Res. 2013 Nov 15;19(22):6173-82.Kim YR., et al. A Bacterial RTX Toxin Causes Programmed Necrotic Cell Death Through Calcium-Mediated Mitochondrial Dysfunction. J Infect Dis. 2013 May;207(9):1406-15. Cheung AK., et al. Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma. Mol Cancer Ther. 2013 Aug;12(8):1393-401.Venturelli S., et al. Differential induction of apoptosis and senescence by the DNA methyltransferase inhibitors 5-azacytidine and 5-aza-2'-deoxycytidine in solid tumor cells. Mol Cancer Ther. 2013 Oct;12(10):2226-36.

细胞心脏病学研究（Cellular Cardiology Research）

Chintalgattu V., et al. Coronary Microvascular Pericytes Are the Cellular Target of Sunitinib Malate-Induced Cardiotoxicity.Sci Transl Med 2013 May; 5(187): 187ra69.

Bornholz B., et al. Impact of human autoantibodies on β1-adrenergic receptor conformation, activity, and internalization. Cardiovasc Res. 2013 Mar 1;97(3):472-80. Wang T., et al. A cardiomyocyte-based biosensor for antiarrhythmic drug evaluation by simultaneously monitoring cell growth and beating. Biosens Bioelectron. 2013 Nov 15;49:9-13.Guo L., et al. Refining the Human iPSC-Cardiomyocyte Arrhythmic Risk Assessment Model. Toxicol Sci. 2013 Dec;136(2):581-94.

Lamore SD., et al. Cellular impedance assays for predictive preclinical drug screening of kinase inhibitor cardiovascular toxicity. Toxicol Sci. 2013 Oct;135(2):402-13.

Doherty KR., et al. Multi-parameter in vitro toxicity testing of crizotinib, sunitinib, erlotinib, and nilotinib in human cardiomyocytes. Toxicol Appl Pharmacol. 2013 May 21. Karen S., et al. The effects of jaspamide on human cardiomyocyte function and cardiac ion channel activity. Toxicology in Vitro. 2013 March; 27(2):745–751.Himmel HM. Drug-induced functional cardiotoxicity screening in stem cell-derived human and mouse cardiomyocytes: Effects of reference compounds. J Pharmacol Toxicol Methods. 2013 Jul-Aug;68(1):97-111.