JIPB | 第68卷第4期已上线 欢迎阅读！

Xiaomin Deng, Shuguang Yang, Qiang Gao, Yanling Chen, Xia Zeng, Minjing Shi, Shaohua Wu, Weimin Tian, Xuchu Wang, Jinquan Chao

https://doi.org/10.1111/jipb.13948

Targeted metabolomic profiling of rubber tree (Hevea brasiliensis) laticifers identified metabolites that were reprogramming by domestication, revealed active isoprenoid metabolism in the laticifers, and discovered loci with potential biosynthetic applications, supporting the potential of developing laticifers as bioreactors for production of valuable metabolites in Hevea.

中文解读：

中国热科院橡胶所/热作全国重点实验室晁金泉团队联合揭示橡胶树乳管细胞次生代谢谱

Qi Yao, Yi Ye, Muyao Yu, Yifu Tian, Qi Liu, Han Zheng, Luqi Huang

https://doi.org/10.1111/jipb.70006

An optimized prime editing system in the model medicinal plant Danshen (Salvia miltiorrhiza) was developed, enabling base transversions, complex insertions and base substitutions. Precise editing of key genes generated germplasm with elevated contents of the bioactive abietane diterpene tanshinone, advancing synthetic biology applications in medicinal plants.

中文解读：

中国中医科学院黄璐琦院士团队以丹参为模式成功优化建立基于PE系统的序列精准插入及替换工具

Xiaotong Wang, Ziyan Xie, Jing Wang, Li Xu, Wei Song, Maolun Gao, Shanshan Chen, Mofan Zhang, Xiaowei Du, Yan Liu, Chunbo Teng, Chengwei Liu, Shilin Chen, Zhichao Xu

https://doi.org/10.1111/jipb.70089

The UGT73 subfamily in Dioscorea nipponica exhibits remarkable catalytic diversity in glycosylation of steroidal saponins. Notably, DnU26 from the UGT73 subfamily and members of the phylogenetically distant UGT91 family independently evolved identical catalytic functions, revealing convergent evolution and the remarkable flexibility of plant glycosyltransferases in generating metabolic diversity.

中文解读：

东北林业大学本草基因组学研究团队揭示穿龙薯蓣甾体皂苷糖基化多样性形成机制

Jiayi Chen, Mingchun Liu, Yang Zhang, Feng Bai

https://doi.org/10.1111/jipb.70077

This mini-review synthesizes recent advances in the biosynthesis and regulation of tomato steroidal glycoalkaloids and discusses the potential applications of metabolic engineering for targeted modulation of steroidal glycoalkaloids.

中文解读：

四川大学生命科学学院张阳团队综述番茄甾体糖苷生物碱的生物合成、调控网络和代谢工程

Yibo Zhang, Dian Jiao, Chenjia Shen, Junhui Zhou, Juan Guo, Jian Yang, Shuang Liu, Ping Su

https://doi.org/10.1111/jipb.70004

Plant prenyltransferases are crucial enzymes catalyzing prenylated natural product biosynthesis. This review examines the structural classification, catalytic mechanisms, enzyme engineering, and heterologous expression of prenyltransferases to advance prenylated natural product-based drug development.

中文解读：

中国中医科学院黄璐琦院士团队对植物异戊烯基转移酶研究进行了系统总结与展望

Wenhua Chen, Yi Shi, Zongyou Lv, Wansheng Chen

https://doi.org/10.1111/jipb.70110

This review explores gene-editing applications in medicinal plants to boost low-yield pharmaceuticals. It covers enhancing agronomic traits and specialized metabolite production, as observed in Salvia miltiorrhiza; discusses limitations and novel solutions, including AI and improved regeneration; and offers perspectives on advancing metabolic engineering for these vital plants.

中文解读：

海军军医大学第二附属医院联合上海中医药大学中药研究所综述药用植物基因组编辑：进展、挑战和前景

Xueqing Fu, Yaojie Zhang, Muyao Yu, Han Zheng, Yuanbing Pan, Pin Liu, Ling Li, Xiaofen Sun, Yuliang Wang, Jingya Zhao, Kexuan Tang

https://doi.org/10.1111/jipb.70133

In medicinal plants, specialized structures such as glandular trichomes serve as microfactories for bioactive compounds. In this review, deciphering how these structures develop and are regulated provides a foundation for precision breeding to enhance the production of high-value plant-derived medicines.

中文解读：

上海交通大学唐克轩教授团队发表药用植物次生代谢物合成器官和组织的特殊结构类型和发育机制研究进展综述

Chao Tan, Shuzhen Men

https://doi.org/10.1111/jipb.70135

This review summarizes current knowledge on plant sterols, encompassing their structural diversity, distribution, biosynthetic pathways, homeostasis, and functions in reproductive and vegetative development.

中文解读：

南开大学门淑珍教授团队系统综述植物固醇的结构多样性、生物合成及其生物学功能

Caibin Zhang, Jingcheng Shi, Rui Deng, Moxian Chen, Alisdair R. Fernie, Youjun Zhang

https://doi.org/10.1111/jipb.70146

This review elucidates how plants utilize metabolons, i.e., dynamic enzyme complexes, to efficiently biosynthesize high-value compounds. An AI-assisted design blueprint is presented for engineering these biosynthetic modules, enabling the programmable production of medicines and nutrients in plants and paving a sustainable path forward beyond traditional agriculture.

中文解读：

中国科学院遗传与发育生物学研究所张有君团队综述植物天然产物生物合成中的代谢区室工程

Ruiqi Zhang, Yuqing Ren, Yuehe Zhao, Hongyan Zheng, Yanzhong Luo, Yuan Liu, Lei Wang, Lan Zhang

https://doi.org/10.1111/jipb.70235

For a century, Vitamin E has been studied for its health benefits. This review traces its journey from basic biological discovery, and the recent identification of its link to chlorophyll degradation, to the modern era of sustainable microbial manufacturing, highlighting the innovative technologies that define its future production.

中文解读：

中国农业科学院生物技术研究所张兰和王磊团队系统总结维生素E研究的百年历程，并绘制下个百年的发展路线图

Federico Scossa, Mustafa Bulut, Thomas Naake, John C. D''Auria, Alisdair R. Fernie

https://doi.org/10.1111/jipb.70236

Similar traits in different organisms may originate from shared ancestry or evolve independently. The terminology used to define phenotypic similarity is often confusing. This review attempts to clarify the definitions and present examples from plant domestication and specialized metabolism to explain how complex traits evolve repeatedly in plants.

Ticao Zhang, Dengli Luo, Guodong Li, Huanchong Wang, Qiang Cao, Rengang Zhang, Yuran Li, Yingan Zhu, Chunhua Ma, Aaron Liston, Hang Sun, Qin Qiao

https://doi.org/10.1111/jipb.70052

High-quality genomes of four wild raspberry species, analysis of their genetic relation ships, identification of centromeres as markers for tracing their hybrid origins, exploration of fruit quality regulation, and discovery of a gene blocking anthocyanin transport and thus causing yellow fruits provides valuable resources for raspberry breeding.

中文解读：

中科院昆明植物所/云南农大等合作解析悬钩子属遗传分化与果实发育机制

Guanhua Zhang, Chunxia Ma, Sheng Wang, Chuanzhi Kang, Chenglong Sun, Wei Liu, Chaogeng Lyu, Xiao Wang, Lanping Guo

https://doi.org/10.1111/jipb.70061

The AMG-LDI-MSI platform uses gold nanoparticle—loaded MoS₂ and doped graphene oxide flexible film substrate combined with laser desorption/ionization for rapid and accurate visualization of a wide range of metabolites in various plant tissues by utilizing a versatile, non-sectioning, matrix-free, dual-ion mode mass spectrometry imaging strategy.

中文解读：

中国中医科学院郭兰萍团队与齐鲁工业大学王晓团队合作开发用于植物多种组织代谢物分析的双极性、高分辨质谱成像新方法

Xinxin Jia, Xiaoliang Zhang, Xueli Chen, Alisdair R. Fernie, Weiwei Wen

https://doi.org/10.1111/jipb.13957

Metabolite genome-wide association studies in tea (Camellia sinensis) identified the 5''-methylthioadenosine/S-adenosylhomocysteine nucleosidase gene CsMTAN. CsMTAN catalyzes the degradation of 5''-methylthioadenosine and S-adenosylhomocysteine, releasing adenine, a precursor in the caffeine biosynthetic pathway. The naturally occurring phenylalanine→tyrosine substitution at residue 179 (F179Y) significantly reduces the catalytic efficiency of CsMTAN.

中文解读：

华中农业大学园艺林学学院闻玮玮团队揭示水平转移的基因CsMTAN重组茶树咖啡因合成的上游生产线

Dan-Dan Liu, Ming-Hui Xu, Lei Zhao, Miao-Yi Li, Zhao-Yang Li, Yuepeng Han, Jian-Ping An

https://doi.org/10.1111/jipb.70001

A module comprising the E3 ubiquitin ligase SINA1, the WUSCHEL-related homeobox transcription factor WOX8, and the transcription factor gene ELONGATED HYPOCOTYL 5 regulates anthocyanin biosynthesis by interacting with the strigolactone signaling repressor SMXL8 and the gibberellic acid signaling repressor RGL3a in apple.

中文解读：

中国科学院武汉植物园联合云南大学揭示SL-GA信号互作及WOX蛋白泛素化调控新机制

Tangli Li, Ming Wang, Huilin Ren, Han Yu, Xiaozhou Luo, Lifei Lv, Huanan Jin, Xiaopu Yin, Rong Chen

https://doi.org/10.1111/jipb.70048

Methylated β-elemene was produced in engineered E. coli using multiple methods, including screening of C-methyltransferases and evolution of germacrene A synthase with UniKP, as well as cofactor engineering. This research expands the chemical diversity of terpenes and highlights the evolutionary plasticity of terpene synthases in generating structural novelty.

中文解读：

杭州师范大学殷晓浦、谌容团队基于人工智能驱动的新酶挖掘和突变设计，实现非常规萜烯甲基-β-榄香烯的生物合成

Xuyang Liu, Shan Jiang, Yawei Li, Liping Gao, Yajun Liu, Jia-Ping Ke, Zhaoliang Zhang, Liang Zhang

https://doi.org/10.1111/jipb.70055

The acylated flavonoid kaempferol-3-O-(6”-p-coumaroyl)-glucoside, a newly characterized compound in tea plants, accumulates mainly in young leaves, is stable during black tea processing, is produced by the acyltransferase CsHCT, and enhances plant cold tolerance, highlighting its dual role in improving tea quality and strengthening stress resilience.

中文解读：

安徽农业大学张梁教授课题组发现CsHCT介导合成山奈酚-3-O-(6〃-对香豆酰基)-葡萄糖苷品质成分

Kun Zhang, Yi-Li Chen, Jia-Ting Lin, Zi-Xin Lu, Yu-Bing Yang, Yong-Kang Li, Chang Yang, Jia Lin, Shuai-Kang Liu, Ling-Yan Wang, Hong-Yun Zeng, Nan Yao

https://doi.org/10.1111/jipb.70081

Phosphorylation fine tunes the activity and stability of the ceramide synthase LOH2, enhancing pathogen resistance by increasing ceramide biosynthesis, salicylic acid accumulation, and pathogenesis-related gene expression.

中文解读：

中山大学姚楠团队揭示神经酰胺合酶的磷酸化调控植物生物胁迫应答的机制

Qun Liu, Zhiqing Wu, Yugang Gao, Yue Xu, Pu Zang, Xinyu Yang, Yan Zhao, Pan Liao, Benoît Boachon, Guoyin Kai

https://doi.org/10.1111/jipb.70130

Auxin signaling regulates the formation of vegetative propagation corms in Gastrodia elata by regulating sugar-acid interconversion, which in turn regulates polysaccharide and starch biosynthesis as well as biosynthesis of the bioactive metabolite gastrodin.

中文解读：

吉林农业大学郜玉钢团队联合浙江中医药大学开国银团队揭示天麻产量和品质提高的GeARF5/GeIAA33-GeSWEET14模块平衡分子机制

Tong Wang, Haofei Wang, Jingna Si, Jiadong Wu, Chen Zhang, Sen Yang, Xuefei Cao, Xiang Zhang, Yan Li, Yousry A. El-Kassaby, Lin Liu, Deqiang Zhang, Zhong Chen, Jianbo Xie

https://doi.org/10.1111/jipb.70176

PagGRF20 is a key regulator of secondary cell wall formation in poplar. PagGRF20 directly promotes cellulose and xylan biosynthesis and represses lignin through interaction with the repressor PagMYB4.

中文解读：

北京林业大学谢剑波团队揭示转录因子通过“转录激活-蛋白互作”双重策略协调木材形成

Haibin Wang, Xumin Ou, Jianing Zhang, Chao Yang, Zhonghui Zhang, Farah Kanwal, Xiaotian Zou, Jun Yang, Shouchuang Wang

https://doi.org/10.1111/jipb.70189

Integrated pangenomic, phylogenetic, and ion mobility mass spectrometry analyses elucidated the evolution of the OMT family, functional divergence of the CCoAOMT clade, and a putative pathway for methoxy-flavonoid biosynthesis in tomato, identifying SlCOMT2 as a key candidate for sakuranetin production.

Hong-Ye Li, Jian-Lin Zou, Bao Nie, Zi-Long Wang, Meng Zhang, Chun-Xue Zhao, Yan-Fang Yang, Xi-Ran Zhang, Xin-Yue Zhan, Li Wang, Min Ye

https://doi.org/10.1111/jipb.70202

Characterization of 31 plant UGT93 glycosyltransferases and their ancestral enzyme showed a catalytic preference for prenylated phenolics and that aromatic and hydrophobic residues in the substrate binding domain are significant for this catalytic preference.

中文解读：

北京大学叶敏团队联合深圳基因组所王丽团队：揭示UGT93家族糖基转移酶对异戊烯基酚的催化偏好性

Zhiyao Wang, Naichao Zhang, Peimin Wu, Fei Chen, Ying Cao, Jing Wang, Yiming Qin, Wenhui Zheng, Yinghua Sheng, Shuai Chen, Baojing Li, Lei Wei, Junli Zhang, Yuqing Liu, Ninghua Tan, Wei Wang, Min Chen

https://doi.org/10.1111/jipb.70216

The O-methyltransferases FsE4MT01 and FsE4MT02, and the glycosyltransferase FsP4''GT catalyze the terminal methylation and glycosylation steps in biosynthesis of the unique antiviral lignan phillyrin in Forsythia suspensa; these enzymes may enable metabolic engineering for enhanced phillyrin production.

中文解读：

破解连翘苷“低产”难题：河南大学陈敏团队解析其生物合成末端关键步骤