3D全自动外泌体荧光检测分析系统基于特异性免疫捕获技术，允许研究者直接分析特定群体的外泌体。通过单分子定位技术成像，可以得到单个外泌体的超分辨成像结果，尺度可以到20nm。全自动外泌体荧光检测分析系统兼容各种生物样本，除了纯化的外泌体之外，对于血液、尿液、恶性肿瘤、腹水中的外泌体也可直接检测分析，大大拓展了研究范围。

应用方向及主要特征

超高分辨单外泌体成像

左：2D单个外泌体成像；右：3D单个外泌体成像

外泌体粒径分析：通过团簇分析外泌体的整体和亚群的粒径分布

膜蛋白表征与共定位分析，以CD63，CD81&CD9为例表征其单阳，双阳和三阳的比例

外泌体内容物表征

对比穿膜处理后外泌体中内容物aGFP的表达

观测细胞中外泌体的分布情况，研究细胞中细胞器或者其他蛋白和外泌体的定位关系

应用案例

法国Abbelight公司开发的3D全自动外泌体荧光检测分析系统是一款无需纯化的、全自动的可对单个外泌体进行表征分析的全新设备。该设备能够提供全方位的单个外泌体表征信息，包括外泌体粒径大小、亚型分布、携带蛋白表达、单个外泌体的膜蛋白与生物标志物共定位等。

应用领域：肿瘤诊断，药系统开发，眼科疾病诊断，疫苗研发，脊髓受伤机制研究，血浆/血清外泌体分析，外泌体工程化，呼吸疾病诊断

测试数据

外泌体2D成像

外泌体3D成像

外泌体形态分析

发表文章

[1] Storci, Gianluca, et al. "CAR+ extracellular vesicles predict ICANS in patients with B cell lymphomas treated with CD19-directed CAR T cells." The Journal of Clinical Investigation 134.14 (2024).

[2] Huang, Xiaowan, et al. "Nanoreceptors promote mutant p53 protein degradation by mimicking selective autophagy receptors." Nature Nanotechnology (2024): 1-9.

[3] Ye, Qian-Ni, et al. "Orchestrating NK and T cells via tri-specific nano-antibodies for synergistic antitumor immunity." Nature Communications 15.1 (2024): 6211.

[4] Shafaq-Zadah, Massiullah, et al. "Exploration into Galectin-3 Driven Endocytosis and Lattices." Biomolecules 14.9 (2024): 1169.

[5] Sanchez-Londono, Mariana, et al. "Visualization of Type IV-A1 CRISPR-mediated repression of gene expression and plasmid replication." Nucleic Acids Research 52.20 (2024): 12592-12603.

[6] Rajbanshi B, Guruacharya A, Mandell J W, et al. Localization, induction, and cellular effects of tau phosphorylated at threonine 217 1[J]. Alzheimer's & Dementia, 2023, 19(7): 2874-2887.

[7] Friedl, Karoline, et al. "Robust and fast multicolor Single Molecule Localization Microscopy using spectral separation and demixing." BioRxiv (2023): 2023-01.

[8] Baschieri, Francesco, et al. "Fibroblasts generate topographical cues that steer cancer cell migration." Science Advances 9.33 (2023): eade2120.

[9] Wessel, Aimee K., et al. "Escherichia coli SPFH membrane microdomain proteins HflKC contribute to aminoglycoside and oxidative stress tolerance." Microbiology Spectrum 11.4 (2023): e01767-23.

[10] Liu, Wei, et al. "Mitofusin-2 regulates leukocyte adhesion and β2 integrin activation." Journal of leukocyte biology 111.4 (2022): 771-791.

[11] Rajbanshi, Binita, et al. "Localization, induction, and cellular effects of tau phosphorylated at threonine 217." Alzheimer's & Dementia (2023).

[12] Pagliuca, M., et al. "38P Single molecule localization microscopy for extracellular vesicles detection in cancer." Annals of Oncology 33 (2022): S1395-S1396.

[13] Robaszkiewicz, A., and K. Gronkowska. "36P EP300 as an epigenetic target in p53 wild-type tumors treated with cisplatin." Annals of Oncology 33 (2022): S1395.

[14] He, Jin, et al. "Heterozygous Seryl‐tRNA Synthetase 1 Variants Cause Charcot–Marie–Tooth Disease." Annals of Neurology (2022).

[15] Gazzola, Morgan, et al. "Microtubules self-repair in living cells." Current Biology (2022).

[16] Liu, Wei, et al. "Mitofusin‐2 regulates leukocyte adhesion and β2 integrin activation." Journal of Leukocyte Biology 111.4 (2022): 771-791.

[17] Portes, Marion, et al. "Nanoscale architecture and coordination of actin cores within the sealing zone of human osteoclasts." Elife 11 (2022): e75610.

[18] Wessel, Aimee K., et al. "Escherichia coli membrane microdomain SPFH protein HflC interacts with YajC and contributes to aminoglycoside and oxidative stress tolerance." bioRxiv (2022).

[19] Radhakrishnan, A. V., et al. "Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration." The Integrin Interactome. Humana, New York, NY, (2021). 85-113.

[20] Jouchet, Pierre, et al. "Nanometric axial localization of single fluorescent molecules with modulated excitation." Nature Photonics (2021): 1-8.

[21] Orré, Thomas, et al. "Molecular motion and tridimensional nanoscale localization of kindlin control integrin activation in focal adhesions." Nature Communications 12.1 (2021): 3104.

[22] Pernier, Julien, et al. "Myosin 1b flattens and prunes branched actin filaments." Journal of cell science 133.18 (2020).

[23] Jimenez, Angélique, Karoline Friedl, and Christophe Leterrier. "About samples, giving examples: optimized single molecule localization microscopy." Methods 174 (2020): 100-114.

[24] Mau, Adrien, et al. "Fast scanned widefield scheme provides tunable and uniform illumination for optimized SMLM on large fields of view." bioRxiv (2020).

[25] Cabriel, Clément, et al. "Combining 3D single molecule localization strategies for reproducible bioimaging." Nature Communications 10.1 (2019): 1980.

[26] Woodhams, Stephen G., et al. "Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain." Pain 160.11 (2019): 2641-2650.

[27] Belkahla, Hanen, et al. "Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances (2019).

[28] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology 4.6 (2019): 972.

[29] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances 4.2 (2018): eaar8082.

[30] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.

用户单位

3D全自动外泌体荧光检测分析系统信息由清砥量子科学仪器（北京）有限公司为您提供，如您想了解更多关于3D全自动外泌体荧光检测分析系统报价、型号、参数等信息，欢迎来电或留言咨询。

客服中心

3D全自动外泌体荧光检测分析系统