TIDES学术海报|30μm硅胶填料在GLP-1及其类似物分离纯化中的应用

Introduction

引言

GLP-1 is a hormone produced mainly by the L-cells of the gut and belongs to the incretin group. At present, many separation method of GLP-1 and its analogs are high performance liquid chromatograph (HPLC) using small silica particle (8/10μm). The separation method using large silica particle (30μm) can effectively improve production efficiency, reduce cost and reduce consumption of organic solvents.

GLP-1是主要由肠道的L细胞产生的激素，属于促胰岛素素类。目前，许多GLP-1及其类似物的分离方法是采用小硅胶颗粒（8/10μm）的高效液相色谱法（HPLC）。采用大硅胶颗粒（30μm）的分离方法可以有效提高生产效率，降低成本，并减少有机溶剂的消耗。

Technical Approach and Highlights

技术方案及亮点

Spherical silica partical with uniform particle size and pore size

The synthetic process of silica can affect the particle size and pore size. Uniform particle size and pore size can increase the effective specific surface area, reduce non-specific adsorption and tailing.

具有均匀粒径和孔径的高纯球形硅胶

硅胶的合成工艺可以影响其粒径和孔径。均匀的粒径和孔径可以增加有效比表面积，减少非特异性吸附和峰拖尾。

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Figure 1. TEM characterization of silica particle

图1.硅胶颗粒的TEM表征

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Figure 2. Pore size distribution diagram

图2. 孔径分布图

Asymmetric bonding

Quality of silica surface group modification directly affects separation effect. The asymmetric bonding technique utilizes the Principle of entropy increase and improves separation effect and loading capacity. 

非对称键合

硅胶表面基团修饰的质量直接影响分离效果。非对称键合技术利用熵增原理，改善了分离效果和负载能力。

Figure 3. Asymmetric C18 has a better separation 

effect when contrasted with C18

图3. 非对称键合与C18对比，非对称C18具有更好的分离效果

Methods

方法

The saperation method is two steps method using large silica partical (Bonnasil-CH C18 30μm 120Å) and small silica partical (Bonnasil-BS C18 Pro 8μm 120Å). For example, a saperation method of Semaglutide is compared with the other two methods in terms of sample loading, purity, yield, time and solvent consumption,etc.

分离方法是采用大颗粒硅胶（Bonnasil-CH C18 30μm 120Å）和小颗粒硅胶（Bonnasil-BS C18 Pro 8μm 120Å）的两步法。例如，以司美格鲁肽为例，将其分离方法与其他两种方法在载样量、纯度、产率、时间和溶剂消耗等方面进行比较。

Figure 4. Chromatogram of Agilebio product

(a), Brand N product (b) and Brand K product (c).

图4. 艾捷博雅产品的色谱图（a），

品牌N产品的色谱图（b）和品牌K产品的色谱图（c）

Results

结果

When 30μm and 8μm silica particle is used for purification of GLP-1 crude product. The loading volume of 30μm silica can reach 7%. The separation time is 1/3 of the polymer. the solvent usage is 1/3 of the polymer. For the secondary preparation of 10μm silica, the loading amount can reach 2%, which is 2 times of other media. The separation time is 1/2 of other products, and the solvent usage is 1/2 of other products. 

当使用30μm和8μm硅胶颗粒纯化GLP-1原料时，30μm硅胶的载样量可以达到7％，分离时间为聚合物的1/3， 溶剂用量为聚合物的1/3。对于10μm硅胶的二次制备，载样量可达到2％，是其他介质的2倍。分离时间为其他产品的1/2，溶剂用量为其他产品的1/2。

Figure 5. The purification results of Agilebio products 

are compared with other brand products.

图5. 艾捷博雅产品与其他品牌产品的纯化结果比较

Conclusion

结论

It can be found by experimental data that this method using large silica particle (30μm) can improve the product loading volume and separation effect, and reduce the separation time and solvent consumption. It can reduce costs and increase efficiency. Therefore, this method is the most suitable for GLP-1 purificatio.

通过实验数据可以发现，使用大硅胶颗粒（30μm）纯化方法可以

①提高产品的装载量和分离效果；

②减少分离时间和溶剂消耗；

③降低成本，提高效率。

因此，这种方法更加适合GLP-1的纯化。

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