LIFA荧光寿命系统附件
LIFA是适用于荧光寿命显微成像（FLIM）。该设备可以利用频域技术在任何宽场显微荧光成像系统获得寿命变化相关图像。系统包含TRiCAM M可调制型像增强CCD相机，可调制光源，调制控制器，定制电脑以及LI-FLIM软件。像增强相机包含像增强器，CCD相机，可以获得*高增益和采集速率。
LIFA荧光寿命系统附件关键特点：
精准的寿命成分：0-300ns的荧光寿命，精度可达30ps RMS
系统技术参数：

Lifetime range	0 - 300 ns
Lifetime resolution	less than 100 ps (single pixel)
Speed	2 lifetime images per second (full frame)
Spatial resolution	26 lp/mm (Gen II); 19 lp/mm (Gen III)
Sensitivity	single photon
Intrascene dynamic range	12 bit
Camera input format	1 inch
Effective pixel size	10.3 μm square (12,9 for lens coupled CCD)

控制器参数：控制器可以经USB线LI-FLIM软件控制

Signal shape	Sinusoidal
Frequency range	1 MHz – 120 MHz
Frequency adjustment resolution	1 kHz
Frequency deviation	100 ppm max.
Harmonics suppression	Better than 25 dB
Output 1 Type a: MultiLED AC signal level DC current adjust	Capable of driving lightsource type a, optionally b and c Adjustable in 250 steps 0 – 1000 mA, 12 bit resolution
Optional lightsource outputs: Type b: Modulated laser diode AC signal level DC level adjust Type c: AOM for CW laser AC signal level Frequency Output 2 AC signal level AC signal after amplification	0.00 – 1.00 V peak-peak 0.00 – 1.00 V 0.00 – 1.00 V peak-peak Half the frequency of output 2 To image intensifier Adjustable from –50 to 0 dBm 15 Vpp nom.
External trigger input	LVTTL (phase shift on falling edge or rising edge)
Impedance	50 Ω
Phase adjustment range	0 - 360° in 1° steps
Phase deviation	< 0.01°
Cathode voltage DC	Adjustable from –150 V to +15 V
MCP voltage (gain) DC	Adjustable from 450 – 1000 V max.
Anode voltage DC	Fixed 6 kV

多LED光源

Peak wavelengths (nm), approximately	405 (CFP), 445 (cerulean, CFP), 470 (GFP), 535 (TRITC), 630 (CY5), others on request
Number of mounted LEDs	3 (standard), or 4 (maximum)
Modulation frequency	up to at least 80 MHz
LEDs switching time	200ms
Coupling	Compatible with existing Hg and Xe microscope adapters
Lifetime drift	less than 30 ps*
Optional Temperature stabilization for long-term (hours) lifetime stability	Active thermoelectric water cooling/heating system
Control temperature range	5 – 45º C
Temperature stability (constant load, constant ambient temp)	± 0.1º C
Dimensions (l x w x h)	346 x 109 x 183 mm
* Measured using stable fluorescent material with single lifetime component of 3.2 ns

多激光光源

Wavelengths (nm)	375 (BFP), 405 (CFP), 445 (cerulean, CFP), 488 (GFP), 515 (YFP, Venus), 647 (CY5), others on request
Number of laser lines	Standard: up to 3 or up to 6; Extreme: up to 4. All combined into one optical fiber output
Output power (mW)	20 mW-300 mW, depending on the wavelength
Modulation frequency	Up to 120 MHz (standard)
Input signal	Digital: 0...1 V / 50 Ω
Control interface	RS232
Coupling	KineFLEX system, with a single-mode polarization maintaining fiber and either FC-APC, FC-PC or FC-P8 output connector
Power stability	Standard: 2%, Extreme: 0.5%
Lifetime drift	Standard: less than 50 ps, Extreme: less than 20-30 ps
Dimensions (l x w x h)	19" rack housing. MultiLASER-3: 480 x 484 x 88 mm; MultiLASER-4 and 6: 610 x 484 x 132 mm
Options	• Multi-channel excitation • Two single-mode optical fiber outputs • Custom fiber outputs on special request
* Measured using stable fluorescent material with single lifetime component of 3.2 ns

TRICAM M相机像增强器指标

CCD相机指标

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LIFA荧光寿命系统附件