分析内容 1. 数据产出统计:对测序结果进行图像识别、去污染、去接头,统计结果包括read长度、read数据、数据产量; 2. RRBS序列与参考序列的比对; 3. Promoter和CpG岛(CGI)覆盖度分析; 4. Promoter和CGI区甲基化分析; 5. 差异性甲基化区域(DMR)分析。 参考文献 [1] Wang L, Sun J, Wu H, et al. Systematic assessment of reduced representation bisulfite sequencing to human blood samples: A promising method for large-sample-scale epigenomic studies. J Biotechnol. 2012. [2] Li S, chowdhry, Liu F et al. Tumor-Suppressive miR148a Is Silenced by CpG Island Hypermethylation in IDH1-Mutant Gliomas. Clin Cancer Res. 2014. [3] Terragni J, Zhang G, Sun Z, et al. Notch signaling genes: myogenic DNA hypomethylation and 5-hydroxymethylcytosine. Epigenetics, 2014.