用数据说话系列之SDA + LTHTh - ICR培养基中和剂的有效性

USP <1116> and the FDA

Aseptic Guide (2004) which states: "Where appropriate, inactivating agents should be used to prevent inhibition of growth by cleanroom disinfectants or product residuals (e.g. antibiotics)".

ISO 14698-1 which points out: "Appropriate additives shall be included to overcome, or minimize, the effects when residual antimicrobial activity at the sampling point is expected".

EN 17141 standard stipulates in its part Culture Media and Incubation the "use of neutralizers in media used for surface sampling to ensure that disinfectant residues on surfaces do not suppress the growth of the microorganisms sampled".

通过下面的实验数据来看看SDA培养基中和剂的必要性吧!

实验模拟最恶劣的消毒剂接触方式进行测试

取120μL或240μL消毒剂直接涂抹到SDA培养基上，待消毒剂与平板充分接触20分钟后，再接种10-100CFU测试菌株，涂布均匀，对照组为不接种消毒剂组，其余操作步骤相同，计算回收率。