美国Hausser scientific血球计数板,霍华德霉菌计数板,准确计算白细胞、红细胞、血小板、组织培养细胞、细菌和精子 The
Hausser Nageotte Bright-Line Hemacytometer allows one to manually count low
leukocyte concentrations below the capabilities of a standard Hemacytometer. The
Nageotte contains two gridded counting areas each containing 40 rectangular
areas. Total volume of each grid is 50 mL for a total volume of 100 mL per
Nageotte. The Nageotte can accurately measure down to 0.1 cell/mL (100 cell/ml).
Cell Depth is 0.5mm . Available with an H-shaped moat(catalog#4000) that
separates the two gridded areas or without a moat (catalog#4001). The Nageotte
Chamber comes with two reusable cover slips(30mm X 33mm X 0.5mm) and 1/2 oz
(approx. 35 pcs.) of disposable cover slips (30mm X 33mm X 0.15mm). PROCESS:
Counting low levels of white cells in white cell-reduced
plateletcomponents. BACKGROUND: Determination of the white cell (WBC) count
in WBC-reduced platelet components requires methods that have a detection limit
in the range of approximately 5.0 x 10(2) to 5.0 x 10(4) per mL. STUDY DESIGN
AND METHODS: With a 50-microL Nageotte hemocytometer and bright-field microscopy
(200x magnification), studies were conducted to develop and validate a method
that could be used routinely with filtered and apheresis-harvested platelets. A
1-in-5 dilution of sample with a commercially available blood-diluting fluid was
used because, with a lower (1-in-2) dilution, the observed number of WBCs was
substantially less than the number expected at relatively high platelet counts
(> 1.9 x 10(9)/mL). RESULTS: The observed and expected WBC counts in
WBC-reduced platelet samples correlated well at levels between approximately 5
and 1100 WBCs per counting area (5.0 x 10(2)-1.1 x 10(5)/mL). At levels of more
than 300 to 400 WBCs per counting area, accurate counts were obtained when 10 of
the 40 rectangles were counted. CONCLUSION: These studies provide data to
confirm that the 50-microL Nageotte hemocytometer can be used to accurately
count low levels of WBCs in platelet components. 货号 产品描述 4000
美国Hausser Nageotte Chamber计数板,含2个cover slips(5400)、1盒5411 4001 Nageotte
Counting Chamber,含2个cover slips(5400)、1盒5411 5400
Reusable盖玻片,30mm×33mm×0.5mm 5411 Disposable Cover
Slips,30mm×33mm×0.5mm,约70片