Cross Section of the Channel: Same Slide – Different Channel Height and Volume
通道的侧面:同样的载玻片不同的通道高度和体积 选择合适的通道进行流体研究
少量培养液,大剪切力下的流体分析
0.1 and 0.2 mm channels
一系列剪切力
0.4 mm channel
控制剪切力大小(<2 dyne /cm²)
0.6 and 0.8 mm channels
静置培养和流体应用
一般规律是 矮的通道更适合流体研究 高的通道更适合静置细胞培养 与染色,固定的溶剂兼容
HUVEC, cultivated over 7 days at 10 dyn / cm². VE-cadherins are stained in green, cell nuclei are stained in blue.
Influence of Shear Stress on Cultured Cells Human umbilical vein endothelial cells (HUVEC) cultured under flow conditions (20 dyn / cm²) in a µ-Slide I 0.4 Luer over 9 days. The primary cells were transduced with the adenoviral vector rAV CMV-LifeAct-TagRFP 24 hours prior to the experiment.