Insplorion传感器涂层、蛋白质表面形纳米颗粒与支持的脂质双层相互作用的影响

支持的脂质双层(SLBs)已被证明是研究蛋白质、肽和纳米颗粒与生物膜相互作用的有价值的模型系统。固体衬底的物理化学性质(例如，形貌、涂层)可能会影响负载型磷脂双层的形成和性质，从而影响随后与生物分子或纳米粒子的相互作用。

       这里,我们用具有耗散监测和NPS技术石英晶体微量天平分析支持涂料(SiO₂ vs Si₃N₄)和表层[用于(纳米等离子体传感(NPS)，嵌入式传感器vs凸式金纳米盘传感器]在负载型磷脂双分子层的形成和与模型蛋白细胞色素c、阳离子聚合裹着的量子点随后的交互的影响。之所以选择特定的蛋白质和纳米颗粒，是因为它们穿透双层膜的程度不同。

       我们发现，双分子层的形成以及随后与细胞色素c的非穿透性关联不受基质成分或形貌的显著影响。相反，纳米颗粒与支持的脂质双层的相互作用取决于基质的组成。纳米颗粒吸附的基质依赖性归因于由二氧化硅支撑的双层膜相对于氮化硅衬底具有更大的负zeta电位，以及包裹纳米颗粒的阳离子聚合物穿透到双层膜。我们的结果表明，纳米级分析物与的支持的脂质双层相互作用程度可能受到底层衬底材料的影响。

原文（英文）：

Influence of Sensor Coating andTopography on Protein and Nanoparticle Interaction with Supported LipidBilayers

Supported lipid bilayers (SLBs) haveproven to be valuable model systems for studying the interactions of proteins,peptides, and nanoparticles with biological membranes. The physicochemicalproperties (e.g., topography, coating) of the solid substrate may affectthe formation and properties of supported phospholipid bilayers, and thus,subsequent interactions with biomolecules or nanoparticles.

Here, we examine the influence ofsupport coating (SiO₂vs Si₃N₄) andtopography [sensors with embedded vs protruding gold nanodisksfor nano plasmonic sensing (NPS)] on the formation and subsequent interactionsof supported phospholipid bilayers with the model protein cytochrome c andwith cationic polymer-wrapped quantum dots using quartz crystal microbalancewith dissipation monitoring and NPS techniques.The specific protein andnanoparticle were chosen because they differ in the degree to which theypenetrate the bilayer.

We find that bilayer formation andsubsequent non-penetrative association with cytochrome c werenot significantly influenced by substrate composition or topography. Incontrast, the interactions of nanoparticles with SLBs depended on the substratecomposition. The substrate-dependence of nanoparticle adsorption is attributedto the more negative zeta-potential of the bilayers supported by thesilica vs the silicon nitride substrate and to the penetrationof the cationic polymer wrapping the nanoparticles into the bilayer. Ourresults indicate that the degree to which nanoscale analytes interact with SLBsmay be influenced by the underlying substrate material.