Agilent的miRNA芯片提供了高通量检测系统的商业化芯片,它具有zei佳的灵敏度和特异性。它使用了革新的标记和探针设计技术,仅需少量样品就可进行实验。
Discover novel pathways of gene regulation
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the activity of specific mRNA targets and play crucial roles in a wide variety of biological processes, including gene expression, development, proliferation, differentiation, and apoptosis. Implicated in human diseases, miRNA expression signatures are an emerging class of molecular biomarkers.
Agilent’s high-performance miRNA Microarray Platform, with its set of optimized reagents and hardware, enables comprehensive miRNA expression profiling across a broad dynamic range, allowing you to confidently elucidate the role that miRNAs play in your research. Exceptionally sensitive and selective oligonucleotide probes enable discrimination between closely related mature miRNAs. Agilent’s SurePrint technology and flexible probe design allow you to easily change and update content to accommodate newly discovered sequences. An efficient direct labeling procedure enables analysis of low total RNA sample input.
Figure
Significance analysis of microarray (SAM) plot showing differentially expressed miRNAs. Most miRNAs were not significant discriminators of class (gray circles), except for six miRNAs that were all significant and all upregulated in the class two tumors (white circles). miRNA, micro-RNA.1
References
1. Micro-RNAs associated with metastasis in uveal melanoma identified by multiplexed microarray profiling. Melanoma Research. 2008 18:184-190.
Agilent 人miRNA芯片13.0
产品性能参数如下:
1. Unrestricted human miRNA microarray (AMADID #021827)
2. 866 human and 89 human viral miRNAs represented
3.Complete content sourced from the Sanger miRBase, v.12.0
4. Coupled probe design and labeling for optimal sensitivity and specificity
5. 8 x 15K slide format printed using Agilent’s 60-mer SurePrint technology
Agilent 小鼠miRNA芯片13.0
产品性能参数如下:
1. Unrestricted mouse miRNA microarray (AMADID #021828)
2. 627 mouse miRNAs and 39 mouse viral miRNA represented
3. Content sourced from the Sanger miRBase, v.12.0
4. Coupled probe design and labeling for optimal sensitivity and specificity
5. 8 x 15K slide format printed using Agilent’s 60-mer SurePrint technology
Agilent 大鼠miRNA芯片
产品性能参数如下:
1. 350 rat miRNAs represented
2. Complete content sourced from the Sanger miRBase, v.10.1
3. Coupled probe design and labeling for optimal sensitivity and specificity
4. 8 x 15K slide format printed using Agilent’s 60-mer SurePrint technology
相关技术文章
Wang H et al. Direct and sensitive miRNA profiling from low-input total RNA. RNA. 13 (1): 151-9, 2007.
Abstract | PubMed |
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