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基因合成服务

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参考报价: 面议 型号: 基因合成服务
品牌: 通用生物 产地: 安徽
关注度: 60 信息完整度:
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AI问答
可以做哪些实验,检测什么? 可以用哪些耗材和试剂?

 

                       公司拥有专业的基因合成服务专家团队和技术设备,整合了先进的基因序列设计和优化技术,可根据客户需求,为您合成任何长度的基因及各种困难基因。
服务订购

基因长度

交货周期

价格(元/bp)

备  注

<400 bp

5~8 工作日

350

 

400~1500 bp

5~8 工作日

1

 

1501~3000 bp

10~15 工作日

1

 

3001~4000 bp

15~20 工作日

1.3

 

4001~5000 bp

20~25 工作日

1.4

 

5001~6000 bp

20~25 工作日

1.4

 

≥6000 bp

商  议

请询价

 

困难基因

商  议

请询价

 

 

◆ 如果客户不指定载体,公司默认把客户目的基因序列克隆到PUC57上,不收取亚克隆费用。


服务优势

周期短

可靠先进的基因合成技术

天衣无缝的无痕克隆方案

精准的表达系统优化

完善的项目管理


服务说明

备注:
1、基因长度:基因序列长度小于300bp的均按300bp的收费。
2、预期克隆载体:请在订单表或服务合同中注明。
3、基因特性
①是否有严重的重复序列:比如一个片段重复若干次;或者100bp内,重复序列长度超过40bp;或者100bp内反向互补序列长度超过30bp。
②基因的GC含量情况:GC含量是否<30%或>70%(常规基因:指基因片段整体或部分的GC含量在30%-70%之间)。

 

服务流程



我公司为客户提供全部技术资料和产品,包括:
-COA文件 -质粒结构图
-约5μg冻干质粒DNA -测序图谱
-含有重组质粒的甘油菌或穿刺菌一管

特别声明:本公司承诺对客户的基因序列不做任何形式的扩散、开放或利用,对相关信息实行完全保密,公司交付产品一个月后,彻底销毁所有相关的引物、质粒和菌种 

Peer-reviewed Citations:
Combined SNPs and miRNAs technologies for beef traceability
http://onlinelibrary.wiley.com/doi/10.1111/jfs.12360/full 
Q Wu, G Zhou, S Yang, X Xu, C Li - Journal of Food Safety ... Premix Ex Taq Kits (Takara Biotechnology Co. Ltd., Dalian, China). Forward primers used for RT-PCR were synthesized by General Biosystems (General Biosystems Co. Ktd., Anhui, China). The forward primer sequences of the... 

Assessment of the fusion tags on increasing soluble production of the active TEV protease variant and other target proteins in E. coli 
https://link.springer.com/article/10.1007/s12010-016-2360-9 
Xuelian Yu, Jiaqi Sun, Weiyu Wang, Li Jiang, Beijiu Cheng, Jun Fan-Applied Biochemistry and Biotechnology ...Primary and secondary antibodies as well as reagents for Western blotting were provided by Sangon Biotech (Shanghai, China). Primer synthesis and DNA sequencing were conducted by General Biosystems (Chuzhou, China)... 

Engineering thermostable (R)-selective amine transaminase from Aspergillus terreus through in silico design employing B-factor and folding free energy calculations
http://www.sciencedirect.com/science/article/pii/S0006291X1632191X 
J Huang, DF Xie, Y Feng - Biochemical and Biophysical Research Communications ...The AT-ATA cDNA sequence containing the NcoI and XhoI restriction sites was synthesized at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and the pET28a(+) expression vector(pET28a(+)-AT-ATA) was constructed as a template for site-directed mutagenesis... 

A Vector Based on the Chicken Hypersensitive Site 4 Insulator Element Replicates Episomally in Mammalian Cells
http://www.ingentaconnect.com/content/ben/cgt/2016/00000016/00000006/art00006
Zhang,Xi;Zhang,Xi;Wang,Xiao-Yin;Wang,Xiao-Yin;Jia,Yan-Long;Jia,Yan-Long;Guo,Xiao;Guo, Xiao;Wang,Yan-Fang;Wang,Yan-Fang;Wang,Tian-Yun;Wang,Tian-Yun-Current Gene Therapy ...trans- gene expression. MATERIALS AND METHODS Vector Construction The cHS4 DNA sequence used in this study was artificially synthesized by General Biosystems, Inc. (Chuzhou, China) [23]. The synthesized sequences... 

TTF1, in the Form of Nanoparticles, Inhibits Angiogenesis, Cell Migration and Cell Invasion In Vitro and In Vivo in Human Hepatoma through STAT3 Regulation
http://www.mdpi.com/1420-3049/21/11/1507/htm 
Bin Xiao, Dongjing Lin, Xuan Zhang, Meilan Zhang and Xuewu Zhang-Molecules …pcDNA3.1 plasmid of STAT3 was synthesized by General Biosystems (An Hui, General Biosystems, Inc., Chuzhou, China)... 

Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells
http://www.nature.com/articles/srep42805 
Chun-Peng Zhao, Xiao Guo, Si-Jia Chen, Chang-Zheng Li, Yun Yang, Jun-He Zhang, Shao-Nan Chen, Yan-Long Jia & Tian-Yun Wang-Sci Rep. …a 2073-bp fragment (position 94–2166) was synthesized by General Biosystems (Chuzhou, China)… 

Genome-wide analysis of Dof family genes and their expression during bud dormancy in peach (Prunus persica)
http://www.sciencedirect.com/science/article/pii/S0304423816305738 
M Chen, X Liu, L Huan, M Sun, L Liu, X Chen, D Gao, L Li-Scientia Horticulturae …All of the primerswere designed using the Beacon Designer 7 software and synthesized by GeneralBiosystems (Anhui, China)… 

Improving thermostability of (R)-selective amine transaminase from Aspergillus terreus through introduction of disulfide bonds
http://onlinelibrary.wiley.com/doi/10.1002/bab.1572/full 
DF Xie, H Fang, JQ Mei, JY Gong, HP Wang, XY Shen, J Huang, LH Mei-Biotechnology and Applied Science … Materials The AT-ATA cDNA from A. terreus sequence, including the NcoI and XhoI restriction sites, was synthesised at General Biosystems (AnHui) Co., Ltd (Chuzhou, China), and plasmid pET28a(+) was used for gene cloning and DNA sequencing... 

Tobacco etch virus protease mediating cleavage of the cellulose-binding module tagged colored proteins immobilized on the regenerated amorphous cellulose
https://link.springer.com/article/10.1007/s00449-017-1772-4 
Xuelian YuJiaqi SunWeiyu WangLi JiangRuyue WangWenjun XiaoBeijiu ChengJun Fan-Bioprocess and Biosystems Engineering ... Materials and methods. Materials. Reagents used in plasmid construction and protein expression were bought from Takara (Dalian, China). Gene and primer syntheses, DNA sequencing are conducted by General Biosystems (Chuzhou, China)...
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