信号通路磷酸化抗体芯片

从细胞膜、胞浆到细胞核，存在多条信号通路串联交叉形成的复杂信号网络。该信号网络在细胞受到胞外刺激后将信号通过级联放大、分散调节等方式传入胞内，引起一系列的综合性细胞应答。一种生物效应的出现往往存在多条信号通路的同步活化，可逆的***酸化修饰反应则是细胞内部zei为普遍和节能的信号蛋白活化调节方式。因此，找到激活的信号通路乃至发生***酸化调变的通路蛋白，往往成生命科学研究的起点。                

信号通路***酸化广谱筛选抗体芯片（PEX100），采用三维高分子膜专利技术，在片基上高密度结合  1318  种高特异抗体，分别检测432个信号蛋白的679个***酸化位点。这些信号蛋白广泛参与多条重要信号通路信号传导过程。芯片针对每一个特定蛋***酸化位点，设置一对抗体分别检测其***酸化（Phospho）和非***酸化（non-Phospho）状态以提高***酸化检测灵敏度和稳定性。一次芯片实验即可实现多条信号通路的同步筛选和具体调变位点的清晰定位，为后续生物现象的深入探索提供明确的研究方向。                

芯片特点：                

• 1. 芯片规格为76 x 25 x 1 mm；

• 2. 实现31条信号通路全面筛选；

• 3. 每种抗体设置2次技术重复；

• 4. 适用于组织、细胞等多类型样本；

• 5. 5x10⁶细胞、200μg总蛋白量即可满足实验；

• 6. 每个检测位点设有***酸化和非***酸化配对抗体；

• 7. 可通用于人、小鼠、大鼠等多类型模式生物检测。

 检测原理：                

检测列表：                

            详细列表下载                

华盈生物还提供针对目标信号通路研究的蛋***酸化抗体芯片：

详情请点击http://www.wayenbio.com/11-205057-75185.html进行查询。

 相关文献：                

1. Luo L L, Zhao L, Wang Y X, et al. ***-like growth factor binding protein-3 is a new predictor of radiosensitivity on esophageal squamous cell carcinoma. Sci Rep-UK, 2015, 5    (中山大学附属肿瘤医院)

2. Zhu Y P, Wan F N, Shen Y J, et al. Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth. Oncotarget, 2015, 6(16): 14488.    （复旦大学附属肿瘤医院）

3. Wang T, Han S, Wu Z, et al. XCR1 promotes cell growth and migration and is correlated with bone metastasis in non-small cell lung cancer. Biochem Bioph Res Co, 2015, 464(2): 635-641.   （上海长征医院）

4. Chen P, Huang H, Wu J, et al. Bone marrow stromal cells protect acute myeloid leukemia cells from anti?CD44 therapy partly through regulating PI3K/Akt–p27Kip1 axis. Mol Carcinogen, 2015 Dec;54(12):1678-85.     (福建医科大学附属协和医院)

5. Wan F, et al. Oxidized low-density lipoprotein is associated with advanced-stage prostate cancer. Tumor Biol, 2015: 1-10.    (复旦大学附属肿瘤医院)

6. Zhu R, et al. pH sensitive nano layered double hydroxides reduce the hematotoxicity and enhance the anticancer efficacy of etoposide on non-small cell lung cancer.Acta Biomater. 2016 Jan 1;29:320-32.    (同济大学)

7. Li W, et al. Withaferin A suppresses the up-regulation of acetyl-coA carboxylase 1 and skin tumor formation in a skin carcinogenesis mouse model.Mol Carcinog. 2015 Oct 16.    (河北大学)

8. Eke I, et al. Simultaneous β1 integrin-EGFR targeting and radiosensitization of human head and neck cancer. J Natl Cancer I, 2015, 107(2): dju419.

9. Lund R R, et al. NADH-cytochrome b5 reductase 3 promotes colonization and metastasis formation and is a prognostic marker of disease-free and overall survival in estrogen receptor-negative breast cancer. Mol Cell Proteomics, 2015, 14(11): 2988-2999.

10. Han B, et al. The role of TGFBI (βig-H3) in gastrointestinal tract tumorigenesis. Mol Cancer,2015, 14(1): 64.

11. Rao W, et al. OVA66 increases cell growth, invasion and survival via regulation of IGF-1R-MAPK signaling in human cancer cells. Carcinogenesis,  April 7, 2014.（上海交通大学医学院）

12. Jia D, et al. Amplification of MPZL1/PZR promotes tumor cell migration through Src-mediated phosphorylation of cortactin in hepatocellular carcinoma. Cell Res, 2014, (24):204-217. (上海仁济医院) 

13. Ding Y, et al. Seed-targeting anti-miR-21 inhibiting malignant progression of retinoblastoma and analysis of their phosphorylation signaling pathways. Exp Eye Res, 2014. (暨南大学) 

14. Dong S, et al. The REGγ Proteasome Regulates Hepatic Lipid Metabolism through Inhibition of Autophagy. Cell Metab, 2013, 18(3): 380-391. （华东师范大学）

15. Li L et al. REGγ deficiency promotes premature aging via the casein kinase 1 pathway.Proc Natl Acad Sci USA.2013; June 13. （华东师范大学）

16. Cui WY et al. Identification and Characterization of Poly(I:C)-induced Molecular Responses Attenuated by Nicotine in Mouse Macrophages. Mol Pharmacol. 2013; 83:61-72. （浙江大学）

17. Eke I et al. EGFR/JIP-4/JNK2 signaling attenuates Cetuximab-mediated radiosensitization of squamous cell carcinoma cells. Cancer Research. 2013; 73(1):297-306.

18. Totonchy JE et al. Aberrant Proliferation in CXCR7+ Endothelial Cells via Degradation of the Retinoblastoma Protein. PLoS ONE. 2013; 8(7): e69828.

19. Xu N et al. Activation of RAW264.7 mouse macrophage cells in vitro through treatment with recombinant ricin toxin-binding subunit B: Involvement of protein tyrosine, NF-κB and JAK-STAT kinase signaling pathways. Int J Mol Med. 2013; 32(3): 729-735. （吉林大学）

20. Li F et al. Superoxide Mediates Direct Current Electric Field-Induced Directional Migration of Glioma Cells through the Activation of AKT and ERK. PLoS ONE. 2013; 8(4): e61195. （第三军医大学）

21. Ranzato E et al. Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair Regen. 2012; 20(5):778-85.

22. Fan H et al. Cardioprotective Effects of Salvianolic Acid A on Myocardial Ischemia-Reperfusion Injury In Vivo and In Vitro. Evid Based Complement Alternat Med. 2012; 508938. （烟台大学）

23. Zhang YM et al. A novel angiogenesis inhibitor impairs lovo cell survival via targeting against human VEGFR and its signaling pathway of phosphorylation. Cell Death Dis. 2012; 3: e406. （西安交通大学）

24. Li Hui et al. Increased prevalence of regulatory T cells in the lung cancer microenvironment: a role of thymic stromal lymphopoietin. Cancer Immunology Immunotherapy. 2011; 60(11): 1587-1596. （天津医科大学）

25. Bernier M et al. Negative regulation of STAT3 protein-mediated cellular respiration by SIRT1 protein. J Biol Chem. 2011; 286(22): 19270-19279.

26. Kang S et al. p90 ribosomal S6 kinase 2 promotes invasion and metastasis of human head and neck squamous cell carcinoma. J Clin Invest . 2010; 120(4):1165-1177.