北京毅新博创生物科技有限公司
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- Identification of Lactobacillus from the Saliva of Adult Patients with Caries Using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry
- Serum Proteomic Profiling for the Early Diagnosis of Colorectal Cancer
- Magnetic Bead-Based Salivary Peptidome Profiling Analysis for Severe Early Childhood Caries
- Salivary biomarkers indicate obstructive sleepapneapatientswithcardiovascular diseases
- Identification of Kininogen-1 as a Serum Biomarker for the Early Detection of Advanced Colorectal Adenoma and Colorectal Cancer
Comparison of Sequencing Platforms for Single Nucleotide Variant Calls in a Human Sample
| 领域： | 蛋白/抗体/蛋白质组,基因/基因组/测序 | ||
| 资料类型： | NONE | ||
下载地址2：Comparison of Sequencing Platforms for Single Nucleotide Variant Calls in a Human Sample
Abstract:
Next-generation sequencings platforms coupled with advanced bioinformatic tools enable re-sequencing of the human genome at high-speed and large cost savings. We compare sequencing platforms from Roche/454(GS FLX), Illumina/HiSeq (HiSeq 2000), and Life Technologies/SOLiD (SOLiD 3 ECC) for their ability to identify single nucleotide substitutions in whole genome sequences from the same human sample. We report on significant GC-related bias observed in the data sequenced on Illumina and SOLiD platforms. The differences in the variant calls were investigated with regards to coverage, and sequencing error. Some of the variants called by only one or two of the platforms were experimentally tested using mass spectrometry; a method that is independent of DNA sequencing. We establish several causes why variants remained unreported, specific to each platform. We report the indel called using the three sequencing technologies and from the obtained results we conclude that sequencing human genomes with more than a single platform and multiple libraries is beneficial when high level of accuracy is required.