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The high-surface area of the (sub)micrometer droplets generated by low-flow nanoelectrospray ionization results in the potential ionization of contaminants present in laboratory air (Volkmer-Engert R.,Schlosser A. J. Mass Spectrom. 2003; 38: 523-525). Neveu1 and coworkers recently reported an active background ion reduction (ABIRD) “bath gas” system designed to reduce the background levels using laboratory air (Proceedings of the 56th ASMS Conference, June 1-5, 2008, Denver, Colorado). Here we report on the implementation of two similar systems for use in combination with both off-line nanospray and on-line nano-LC/MS.