诚信认证:
工商注册信息已核实!样品: | 蛋白质 | 项目: | 蛋白质质谱分析 |
方案文件名 | 下载 |
---|---|
Nano LC-MS流路的高压可视连接 |
下载此篇方案 |
Nanobore reversed-phase HPLC has become a mainstay for protein identification by modern tandem mass spectrometry. Enhanced robustness and versatility is obtained with trapping columns for on-line preconcentration and desalting of samples. Trapping columns of varying sizes and packing materials are available, yet few configurations are available for 75 μm internal diameter (ID) packed-tip columns frequently employed in high-sensitivity work. Using a novel fluoropolymer and PEEK™-based zero-dead-volume fitting with packed-tip, silica-fritted capillary columns, variable trap column geometries, and column chemistry combinations were investigated toward optimizing chromatographic performance in a typical nanobore RP-HPLC-MS system.