仪器简介 IngenyPhorU是荷兰Ingeny公司基于其专利U形隔离装置专门为DNA突变分析设计的DGGE垂直电泳仪，是进行DNA突变分析的zei佳工具。IngenyPhorU系统用来进行DGGE十分方便，这种技术能检测到近100%的突变。IngenyPhorU同样也适用于其他垂直电泳技术，如CDGE(持续变性凝胶电泳)，SSCP(单链构造多形态分析)，HA(异源双链核酸分析)和PTT(蛋白切断实验)等。温度是DGGE实验的关键因素，IngenyPhorU使用17L大容量缓冲液，精确控制温度变化保持在0.1°C内。通过另购外置式冷却器，能将标配的RT+5~70°C扩展至0~70°C，适应有低温需求的实验。 指标参数 尺寸（长×宽×高） 56cm×26cm×41cm zei小缓冲液体积 17 L 温度范围 0-70℃（需另外选配外置式冷却器） 控温精度 <0.1℃ 凝胶规格 28cm×18cm zei大检测孔数 96 梳子 20孔，32孔，48孔 220V-240V，50Hz 或 110V-130V，50/60Hz 仪器特点   ·96孔容量，每跑一次电泳可分析一个微孔板的样品 ·制胶过程中保证100%无泄漏 ·高重复性的制胶系统可以提高凝胶间的可比性 ·精确的温度控制，结果的重复性好 ·耐腐蚀的钛接线端提高使用寿命 ·凝胶装置中保持温度高一致性 ·更长的凝胶可以在每个泳道中分离更多片段 ·使用更高质量的电极（铂-铱合金） ·可以获得DGGE引物，检测人类基因的突变   耐腐蚀的钛接线端 IngenyPhorU的应用 CDGE（持续变性凝胶电泳） SSCP（单链构造多形态分析） HA（异源双链核酸分析） PTT（蛋白切断实验） DGGE（变性梯度凝胶电泳） TGGE（温度梯度凝胶电泳） 检测CFTR基因突变     检测致癌基因RET突变 检测TP53外显子突变   IngenyPhorU的常用领域 人类基因突变检测 检测线粒体基因突变 检测从泥土，新鲜水或盐水中得到的细菌样品生物多样性 检测肠道细菌群的生物多样性 法医鉴定中线粒体DNA检测 HLA基因组织分型 基因组差异分析 植物科学中家谱分析 RAPD-DGGE检测植物基因组的多形性 在更多领域中的应用正不断被发现

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Aakvik T., et.al. 2009, A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species

Allgaier M., Grossart H.P. 2006, Seasonal dynamics and phylogenetic diversity of free-living and particle-associated bacterial communities in four lakes in northeastern Germany

Jolanda K. Brons and Jan Dirk van Elsas, 2008, Analysis of Bacterial Communities in Soil by Use of Denaturing Gradient Gel Electrophoresis and Clone Libraries, as Influenced by Different Reverse Primers

Cook A.A., et.al. 2005, Denaturing gradient gel electrophoresis (DGGE)as a tool for identification of marine nematodes

Dadheech P.K., et.al. 2009, Molecular detection of uncultured cyanobacteria and aminotransferase domains for cyanotoxin production in sediments of different Kenyan lakes

Dilly O., et.al. 2003, Bacterial Diversity in Agricultural Soils during Litter Decomposition

Dooms S., et.al.2006,Denaturing Gradient Gel Electrophoresis (DGGE) as a tool for the characterisation of Brachionus sp. strains

Duineveld B.M., et.al.1998, Analysis of the Dynamics of Bacterial Communities in the Rhizosphere of the Chrysanthemum via Denaturing Gradient Gel Electrophoresis and Substrate Utilization Patterns

Federici E., et.al.2007, Addition of allochthonous fungi to a historically contaminated soil affects both remediation efficiency and bacterial diversity

Geets J., et.al. 2005, DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria

Gich F., et.al. 2005, Specific Detection, Isolation, and Characterization of Selected, Previously Uncultured Members of the Freshwater Bacterioplankton Community

Green S.J., et.al. 2008, A salinity and sulfate manipulation of hypersaline microbial mats reveals stasis in the cyanobacterial community structure

Hamberger A., et.al.2008, Anaerobic Consumers of Monosaccharides in a Moderately Acidic Fen

Harrington C.R., et.al.2008, A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities

Hendrickx B., et.al.  2005, Dynamics of an Oligotrophic Bacterial Aquifer Community during Contact with a Groundwater Plume Contaminated with Benzene, Toluene, Ethylbenzene, and Xylenes: an In Situ Mesocosm Study

Köpke B., et.al. 2005, Microbial Diversity in Coastal Subsurface Sediments: a Cultivation Approach Using Various Electron Acceptors and Substrate Gradients

R. Laforgue, et.al.2009 , Evaluation of PCR-DGGE methodology to monitor fungal communities on grapes

Lam C., and Harder T. 2007, Marine macroalgae affect abundance and community richness of bacterioplankton in close proximity

Landy E.T., et.al.2007, Bacterial diversity associated with archaeological waterlogged wood: Ribosomal RNA clone libraries and denaturing gradient gel electrophoresis (DGGE)

J. R. Lawrence, et.al.2008, Community-Level Assessment of the Effects of the Broad-Spectrum Antimicrobial Chlorhexidine on the Outcome of River Microbial Biofilm Development

Leys N., et.al.2003, Occurrence and Phylogenetic Diversity of Sphingomonas Strains in Soils Contaminated with Polycyclic Aromatic Hydrocarbons

Malin C., and Illmer P. 2007, Ability of DNA content and DGGE analysis to reflect the performance condition of an anaerobic biowaste fermenter

Manefield M., et.al.2002, RNA Stable Isotope Probing, a Novel Means of Linking Microbial Community Function to Phylogeny

Manske A.K., et.al. 2007,Subfossil 16S rRNA Gene Sequences of Green Sulfur Bacteria in the Black Sea and Their Implications for Past Photic Zone Anoxia

Martínez-Iñigo M.J., et.al.2009, Bulk soil and rhizosphere bacterial community PCR–DGGE profiles and β-galactosidase activity as indicators of biological quality in soils contaminated by heavy metals and cultivated with Silene vulgaris (Moench) Garcke

McAuliffe L., et.al. 2003, Differentiation of Mycoplasma Species by 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis Fingerprinting

Muyzer G, et.al.1993, Profiling of Complex Microbial Populations by Denaturing Gradient Gel Electrophoresis Analysis of Polymerase Chain Reaction-Amplified Genes Coding for 16S rRNA

de Oliveira V.M., et.al.2005, A ribosomal RNA gene intergenic spacer based PCR and DGGE fingerprinting method for the analysis of specific rhizobial communities in soil

Payne M.S., et.al.2006, Microbial Diversity within Early-Stage Cultured Panulirus ornatus Phyllosomas

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Qvit-Raz N., et.al. 2008, Drop-Size Soda Lakes: Transient Microbial Habitats on a Salt-Secreting Desert Tree

Rehman H., et.al. 2007, Effects of Dietary Inulin on the Intestinal Short Chain Fatty Acids and Microbial Ecology in Broiler Chickens as Revealed by Denaturing Gradient Gel Electrophoresis

Rosado, A.S., et.al. 1998, Genetic Diversity of nifH Gene Sequences in Paenibacillus azotofixans Strains and Soil Samples Analyzed by Denaturing Gradient Gel Electrophoresis of PCR-Amplified Gene Fragment

Tkáciková L, et.al. 2006, PrP gene polymorphism in sheep breeds in Slovakia and susceptibility to scrapie.

Uyttebroek M., et.al. 2007,Characterization of Cultures Enriched from Acidic Polycyclic Aromatic Hydrocarbon-Contaminated Soil for Growth on Pyrene at Low pH 

Wang H., et.al. 2008, Impact of crop species on bacterial community structure during anaerobic co-digestion of crops and cow manure

Weinert N., et.al. 2009, Molecular fingerprints reveal that the effects of genetically modified potato plants with increased zeaxanthin content on bacterial and fungal rhizosphere communities do not exceed natural cultivar variability

Winter C., et.al. 2009, Seasonal changes of bacterial and archaeal communities in the dark ocean: Evidence from the Mediterranean Sea

Worthley D.L., et.al.2009, A human, double-blind, placebo-controlled, crossover trial of prebiotic, probiotic, and synbiotic supplementation: effects on luminal, inflammatory, epigenetic, and epithelial biomarkers of colorectal cancer

Yan Q.Y., et.al. 2006, Genetic Diversity of Plankton Community as Depicted by PCR-DGGE Fingerprinting and its Relation to Morphological Composition and Environmental Factors in Lake Donghu

Zijnge V., et.al.2006, Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology

Zouache K., et.al. 2009, Persistent Wolbachia and Cultivable Bacteria Infection in the Reproductive and Somatic Tissues of the Mosquito Vector Aedes albopictus

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