高通量LC-MS/MS技术定量分析全血中的免疫抑制剂

前言：

　　Immunosuppressant drugs inhibit the body’s immune system and are used in organ transplant patients to prevent organ rejection. Liquid chromatography-mass spectrometry (LC-MS/MS) is a widely-accepted technique for the determination of immunosuppressant drugs in whole blood by research laboratories.

　　This application note describes a fast, sensitive, reliable, and accurate LC-MS/MS quantitative method for use by research laboratories for the simultaneous analysis of tacrolimus, sirolimus, everolimus and cyclosporin A in whole blood.

仪器：

Thermo Scientific TSQ Quantum mass spectrometer

结论：

　　A fast, sensitive, reliable and accurate method was developed for the quantification of tacrolimus, sirolimus, everolimus and cyclosporin A in whole blood by research laboratories. The use of column multiplexing technology allows for a 1 min analytical method, which enhances sample throughput.