使用Finnigan公司TSQ Quantum Ultra AM进行药品混合物的精确质量测定

摘要：

Accurate mass measurements are used to propose elemental compositions for compounds under investigation, utilizing high-resolution to reduce the number of interfering isobaric materials.

To obtain accurate mass measurements on the Finnigan TSQ Quantum Ultra AM, a data dependent, Selected Ion Monitoring (SIM) scan is performed using reference lock masses. This accurate mass SIM mode can yield greater sensitivity than a full scan MS analysis by spending significantly more time sampling the actual ion of interest, resulting in improved ion statistics across the mass of interest.

The procedure for accurate mass determination on the Finnigan TSQ Quantum Ultra AM involves linearizing the mass range using a calibration solution, then refining the accurate mass measurement using reference lock masses.

仪器：

Finnigan TSQ Quantum Ultra AM

结论：

This report illustrates the simple procedure that can be used to identify and mass measure target compounds or unknown compounds in a complex mixture, using the unique data dependent AutoSIM and AutoLock functions on the Finnigan TSQ Quantum Ultra AM. The AutoSIM procedure successfully identified all eight components in the mixture, and mass measured them to within an accuracy of 4 ppm. This was within the mass accuracy measurements obtained for the same test mixture using a commercially available quadrupole – time of flight geometry instrument.

The accurate mass AutoSIM procedure can be used in a data dependent scan mode, eliminating the need to know the target mass of an analyte before analysis. Accurate mass measurements can be determined for unknown compounds in a complex mixture, simplifying the identification of these components.