使用线性离子阱质谱分析口服液中多种违禁药物、美沙酮及其代谢物

前言：

Traditionally, the analysis of urine samples has been the major approach for the monitoring of drugs of abuse.However, a common risk for this type of analysis is adulteration or manipulation of the sample at the pointof collection. As an alternative, the analysis of oral fluid provides an easy method of sample collection and has the advantage of providing a relatively clean matrix. Because of the reduced sample volume this technique requires a high sensitivity and robust analytical method to make saliva/oral fluid-based diagnostics an attractive alternativeto conventional methods.

In this report, a rapid and rugged LC-MS/MS method using the Finnigan LXQ is described for analyzing a mixtureof twenty drugs and their metabolites using intelligent automated mass spectrometry (INTAMS). The detectionlimits for the mixture of drugs and dynamic range are superior to results reported previously.2 In addition, thismethod provides for the simultaneous identification and quantification of drugs and their metabolites.

仪器：

Surveyor Plus

LXQ linear ion trap mass spectrometer

结论：

Rigorous simultaneous characterization and quantification of a large number of drugs and their metabolites in a biological matrix can be performed in a fast and robust LC/MS/MS method using a Finnigan LXQ linear ion trap mass spectrometer. The superior sensitivity and faster cycle time of the LXQ makes this possible in a single chromatographic run, resulting in high throughput analyses. High specificity quantification was done using MS³ data which can reduce overall chemical noise even if there is a co-eluting isobaric interfering ion. Additional compound confirmation was obtained using Mass Frontier, where a high match score to a library search provided enhanced confidence in the compound identification.