UHPLC-MS/MS同时分析饮料中的水溶性维生素

简介：

Vitamins are nutrients essential to human health, and are present in almost all types of foods. In addition to food sources, vitamin supplements are often consumed to ensure adequate vitamin intake, as a customary diet does not always provide sufficient vitamins due to bias and/or limitation in the choice of foods, or malfunctions in digestion and ingestion. Vitamin supplements are available in various forms such as single- or multivitamin tablets, formula, and vitaminenriched beverages (VEB). Certain foods are commercially fortified with vitamins and/or other nutritional essentials such as minerals. Based on their solubility, vitamins are divided into two categories: water-soluble vitamins (WSV) and fat-soluble vitamins (FSV). WSVs include vitamin C (ascorbic acid), B1 (thiamine), B2 (riboflavin), B3 (niacin, niacinamide), B5 (pentothenic acid), B6 (pyrodoxine), B7 (biotin), B9 (folic acid), and B12 (cyanocobalamine). Accurate quantitative measurements for vitamins are required to ensure product quality and regulatory compliance as well as to monitor vitamin intake.

LC methods are generally used for simultaneous determination of multiple vitamins of interests and for establishing vitamin profiles in a variety of matrices with various modes of detection.7–10, 13–16 Here we present a high throughput method for simultaneous determination for the above mentioned ten WSVs using ultrahigh-performance LC and tandem mass spectrometry (UHPLC-MS/MS). Chromatography was optimized for the total resolution of all target analytes on a Thermo Scientific Dionex Acclaim® C30 reversed-phase (RP) column. An MS/ MS instrument was operated in selected reaction monitoring (SRM) mode for the best selectivity and sensitivity, and an isotope labeled internal standard (IStd) was used for accurate quantitation.

Randomly selected VEBs were assayed by this method for the selected vitamins, and when compared with product labeling, much higher values were observed for most of the vitamins.

仪器：

Dionex UltiMate® 3000 RSLC

Thermo Scientific TSQ Quantum Access MAX™ MS/MS instrument

结论：

This study describes a UHPLC-MS/MS method for simultaneous quantitation of WSVs in beverages. This method demonstrated excellent correlation of determination, precision, accuracy, and selective and sensitive detection with low quantitation limits. This method was successfully applied to the determination of WSVs in beverages and energy supplements with presented results.