以1000个蛋白每小时的速度鉴定大肠杆菌酶解产物中的蛋白质

简介：

Enormous improvements in mass spectrometry technology over the last few years have allowed for ever deepening analysis of complex proteomes. However, many low abundance proteins remain undetected in current large-scale proteomic analyses due to the limits on the rate at which MS/MS spectra can be acquired. To solve this undersampling problem, we developed the Thermo Scientific LTQ Orbitrap Velos, a new hybrid linear ion trap-Orbitrap mass spectrometer that provides faster MS/MS scanning in the ion trap (up to 10 peptide sequences per second), thereby increasing the depth of analysis of complex protein mixtures.

This application note demonstrates and discusses the ability of the newly developed hybrid instrument to identify more proteins, with increased sequence coverage and confidence. Different strategies regarding chromatographic run times and utilizing the instruments various dissociation techniques are compared.

仪器：

Thermo Scientific LTQ Orbitrap Velos mass spectrometer

总结：

The identification of proteins from complex mixtures requires high sensitivity as well as high acquisition speed.The technological advancements implemented in the LTQ Orbitrap Velos mass spectrometer with more than twice the scan speed of an LTQ Orbitrap XL mass spectrometer, help to overcome undersampling, increasing the depth of the analysis of complex proteomes. The enhanced sensitivity and improved duty cycle of the new instrument enables a dramatic increase of the number of unique peptide IDs, which translates into an increased number of proteins identified with higher sequence coverage and confidence.The LTQ Orbitrap Velos mass spectrometer

• provides an increased scan speed in MS and MS/MS mode due to its higher transmission ion source, dual pressure ion trap and improved HCD cell.

• identifies more than 1,000 proteins per hour from a proteolytic digest of E. coli whole cell lysate.

• provides a higher HCD scan speed and much improved spectral quality opening up new perspectives for de novo sequencing, quantitation of chemically labeled samples, and analysis of modifications which require high mass accuracy.

• offers an increase in experimental throughput, confidently identifying more peptides and proteins than any other mass spectrometer currently on the market.