LC-MS/MS数据关联模式下使用0.5ppm质量精度的SIM鉴定

　　简介：Purpose: Combination of dynamic range enhancement andimprovement of mass accuracy for protein identification in complex mixtures

　　Methods: Nano-LC-MS and nano-LC-CID-MS/MS using a Hybrid Linear Ion Trap–FTICR Mass Spectrometer (Thermo Scientific LTQ FT)

　　Results: Demonstration of high dynamic range with high mass accuracy (<0.5ppm) which greatly improves the coverage and identification of proteins using a protein mixture.

　　仪器：Nano-LC-MS and nano-LC-CID-MS/MS

　　结论：

　　• The described method setup offers mass accuracy of better than 0.5 ppm for the precursor ion;

　　• Improved dynamic range by accumulating more ions (10-fold increase) for the FT master scan;

　　• Improved dynamic range results in more identification with higher sequence coverage;

　　• SIM scan in the ICR cell and the MS/MS experiment in the linear trap are performed in parallel;

　　• Shorter database search times due to excellent mass accuracy;

　　• The probability of false positive hits is greatly reduced.