赛默飞色谱与质谱分析
400-6694-175
分析测试百科网 认证会员，请放心拨打！
使用线性离子阱-Orbitrap组合质谱的高能碰撞诱导解离和数据关联技术分析磷酸化肽段
发布时间：
2011-07-01 17:36
来源：
赛默飞色谱与质谱分析
| 方案文件名 | 下载 |
|---|---|
使用线性离子阱-Orbitrap组合质谱的高能碰撞诱导解离和数据关联技术分析磷酸化肽段 |
下载此篇方案 |
简介：
To demonstrate the benefits of Higher Energy Collision Induced Dissociation (HCD) applying Data Dependent™ methods under nano LC time scales.
仪器：
Thermo Scientific LTQ Orbitrap XL ETD
结论：
Even on an LC time scale, HCD generates excellent fragmentation results with respect to both low mass range information and overall fragmentation yield. This is especially pronounced in MS/MS experiments of phosphopeptides which typically fragment significantly more using HCD than CID. HCD spectra provide fragment information over the entire mass range of the peptide.
