快速GC-HRMS定量分析16种EC规定PAH化合物

Introduction:

The European Commission Regulation (EC) No 208/2005 of February 4, 2005, which came into force on April 1, 2005, provides maximum levels for benzo pyrene in different groups of food of which have the strongest regulation foods for infants and young children with max. 1.0 μg/kg and smoked meats and smoked meat products with max. 5.0 μg/kg[1].

During GC/MS method setup it turned out that single quadrupole desktop MS instruments could not provide the necessary selectivity at the low decision level.

The quantitation was done using isotope dilution technique by the addition of isotopically labeled and fluorinated standards before extraction, as well as for the determination of the response factors of all PAH under investigation. Recovery values have been determined by the addition of three 2H labelled compounds.

Instrument:

Thermo Scientific DFS High Resolution GC/MS system coupled to a Thermo Scientific TRACE GC Ultra™ gas chromatograph

Conclusions：

The retention time of of more than 90 minutes for regular chromatography conditions was successfully reduced to approximately 25 minutes maintaining chromatographic resolution. In practice the fast GC separation combined with a high resolution GC/MS detection system has proven to be a fast and reliable quantitation of PAH at the legally required level in routine analysis.