LC-MSMS分析水中的氯乙基酰苯胺类农药

　　Abstract:

　　Recently, the U.S. Environmental Protection Agency (EPA) promulgated Method 535 entitled “Measurement of Chloroacetanilide and Other Acetamide Herbicide Degradates in Drinking Water by Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS).”1 This method outlines a technique for the analysis of the fate of chloroacetanilide herbicide compounds in drinking water, specifically ethanesulfonic acid (ESA) and oxanillic acid (OA) degradates.

　　The degradates of chloroacetanilide herbicide compounds are polar, making them ideal compounds for analysis by negative electrospray ionization mass spectrometry. This application note illustrates the direct analysis of drinking water spiked with chloroacetanilide herbicide compounds. Detection limits for these compounds are below the lowest required calibration levels outlined in the EPA’s method, eliminating the need for time consuming sample preparation. Excellent linearity and reproducibility are demonstrated over the entire calibration range.

　　Instrument：

　　EQuan™ 环境定量系统

　　Accela 高效液相色谱

　　Conclusion：

　　This application note compared a slightly modified method to the EPA’s method 535. Significant time savings were achieved by bypassing offline solid phase extraction and preconcentration. The sensitivity of the assay was also higher

　　than the EPA method, and there is the potential for even higher sensitivity and time savings in this method if the

　　EQuan™ online preconcentration system was used in conjunction with the Accela Fast HPLC chromatographic system.