赛默飞色谱与质谱分析

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2018年10期的《食品安全质量检测学报》中刊登的,“馥郁香型白酒等级鉴别的研究......

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在全解决方案中使用新的样品制备技术,改进蛋白酶解和多肽定量的速度及重现性

发布时间: 2015-10-15 10:44 来源: 赛默飞色谱与质谱分析

下载地址: 在全解决方案中使用新的样品制备技术,改进蛋白酶解和多肽定量的速度及重现性


  To describe a rapid and precise protocol for peptide analysis utilizing immobilized, heat stable trypsin and micro-elution solid-phase extraction (SPE) coupled with next generation ultra-high pressure liquid chromatography and high resolution mass spectrometry (UHPLC-HRMS). Traditional trypsin digest protocols are time consuming, with some protocols taking up to one and a half days to complete and involving multiple steps including evaporation and reconstitution. This adds potential for a high degree of variability. The Thermo Scientific™ SMART Digest™ kit was used to digest cytochrome C with a 10 minute digestion protocol. This was followed by Thermo Scientific™ SOLAμ™ SPE plates to clean and concentrate the digest prior to injection onto the Thermo Scientific™ Vanquish™ UHPLC system. Following separation on a Thermo Scientific™ Acclaim™ C18 RSLC analytical column, high-resolution mass spectrometry was performed using a Thermo Scientific™ Q Exactive™ Plus hybrid quadrupole-Orbitrap mass spectrometer. A high-throughput, reproducible workflow was achieved that can be applied to nontargeted, semitargeted, or targeted quantitative environment for peptide analysis.


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