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快速pH梯度离子交换色谱荧光检测用于单克隆抗体药物电荷变异体的高灵敏度分析

发布时间: 2015-09-24 15:40 来源: 赛默飞色谱与质谱分析

下载地址: 快速pH梯度离子交换色谱荧光检测用于单克隆抗体药物电荷变异体的高灵敏度分析


  Fractions of antibodies obtained by size exclusion chromatography require a relatively large injection volume on an ion exchange column, to detect analyte by UV absorption. However, those fractions contain a large amount of salt. The combination of large volume and high salt concentration results in peak breakthrough and jeopardizes characterization and quantification. The superior sensitivity of fluorescence detection compared to UV allowed to reduce the sample volume and eliminated breakthrough effects caused by the salt–rich sample matrices.


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