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高速LC分析辛伐他汀片
| 资料文件名 | 下载 |
|---|---|
HPLC-PDA-药物的液相检测Analysis of Simvastatin Tablets by High Speed LC.pdf |
下载此篇资料 |
前言：
The USP method for Simvastatin Tablets (30-NF25) employs HPLC with UV detection at 238 nm.2 Simvastatin elutes at about 10 min on a 250 mm × 4.6 mm L1 column with an isocratic mobile phase containing 35:65 (v/v) 38 mM phosphate buffer (pH 4.5): acetonitrile flowing at 1.5 mL/min. The USP method requires the chromatographic performance to meet the following criteria:
capacity factor (k’) >3; Efficiency (N, no. plates) > 4500; Asymmetry < 2.0; Precision (Peak area %RSD, n = 3), 2.0%. For a definition of these parameters, see Reference 2.
仪器：Accela™ High-Speed Liquid Chromatography system
结论：
The Accela High Speed Chromatography system equipped with a Hypersil GOLD 1.9 μm column increases the throughput of a Simvastatin Tablet assay 10-fold with performance that exceeds the USP requirements. The conditions required for the high-speed method are conveniently calculated by using a web-based Method Transfer calculator or simple formulas for flow rate and injection volume.
