分析测试百科网 认证会员，请放心拨打！
采用电喷雾检测器HPLC和梯度离子对试剂测定硫酸庆大霉素含量
| 资料文件名 | 下载 |
|---|---|
Dionex-CAD检测器-离子对柱子检测庆大霉素A Gentamicin Sulfate Assay Using HPLC Charged Aerosol Detection with an Ion-Pairing Reagent Gradient.pdf |
下载此篇资料 |
前言：To develop a direct-detection gentamicin assay that eliminates o-phthalaldehyde, as used in the U.S. Pharmacopeia (USP) monograph1 or other derivatization methods.
Methods: Using UHPLC and a Thermo Scientific Dionex Corona ultra RS Charged Aerosol Detector, an assay is developed to separate the congeners in gentamicin sulfate and gentamicin-sulfate-containing products with a mobile phase comprised of lower ionpairing reagent concentrations as compared to many previously published methods.
结论： Assay of four samples with three different matrices gave results that were equal to the product label claims.
