SIEVE代谢组差异分析软件

SIEVE software compares LC/MSn spectra from healthy and disease (or control versus treatment) samples to find differentially expressed features. Trend analysis can be preformed to detect changes associated with dosage effects or time points, enabling comparison of multiple classes of samples. Thanks to the pre-filtering of the SIEVE software, only peptides or metabolites that show statistically significant differences in abundance between sample sets or over time need be identified through the incorporated search capabilities.

Key Features and Benefits

• Uses the MS intensities from raw LC/MS data to find statistical differences, eliminating the need to manipulate or model peaks
• Can compare hundreds of LC/MS experiments at a time, but can also perform a simple comparison of two sample files
• Performs trend analyses to detect changes associated with dosage effects or time points
• Employs a novel algorithm called ChromAlign to reduce the effect of chromatographic variability between samples
• Determines a p-value for the expression ratio of each putative biomarker, providing an extra measure of confidence
• Incorporates individual search algorithms for peptide and metabolite data
• Accommodates high-resolution and low-resolution data generated on the Thermo Scientific instruments, including data from LTQ, LTQ Orbitrap, and Exactive mass spectrometers