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UHPLC联合电雾式检测器通过电荷、大小和异构性,无需标物分析多聚糖

发布时间: 2015-03-18 17:21 来源:赛默飞色谱与质谱分析
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UHPLC联合电雾式检测器通过电荷、大小和异构性,无需标物分析多聚糖.pdf

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  Glycoproteins of biological, diagnostic or therapeutic interest owe key aspects of their normal function to the oligosaccharides attached to the protein backbone. Changes in the number, type, composition or linkage pattern of these glycans may serve as a biomarker of disease or influence the efficacy of a biotherapeutic product.1 For this reason, the ability to correctly identify and measure these glycans is of scientific interest, and to do so reliably, quickly and inexpensively is of practical benefit. This work explores direct detection of native glycans as an alternative to the common techniques for glycan analysis that rely on derivatization reactions to render glycans detectable. The lack of a detectable chromophore in native glycans is overcome by using HPLC with charged aerosol detection, a detector that can quantitatively measure any non-volatile compound.

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