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CyTOF 2质谱流式细胞仪

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参考报价: 面议 型号: CyTOF 2
品牌: 富鲁达 产地: 富鲁达
关注度: 3 信息完整度:
样本: 暂无样本 典型用户: 暂无
产地属性美洲仪器种类流式细胞仪
应用系统科研型流式分析分选系统

The CyTOF® 2 system, the world’s most advanced mass cytometry platform, brings high-parameter capabilities to protein research, providing an unparalleled capacity to generate high-resolution phenotypic and functional profiles of cells from normal and diseased states.

The CyTOF 2 mass cytometer analyzes individual cells labeled with stable metal isotopes using state-of-the-art inductively coupled plasma time-of-flight technology. With more than 120 detection channels, the CyTOF 2 has the capability to simultaneously resolve multiple elemental probes per cell at high acquisition rates, thereby maximizing the per-cell information obtained from a single sample.

Mass cytometry employs elemental tags that are uncommon in biological systems. Cells stained with metal-conjugated probes in a single-cell suspension are introduced into the CyTOF 2 and undergo a multistep process within the instrument to detect these metal tags and record the identity and amount of each probe on each cell.

CyTOF 2
Specification

Channels121
Mass Range89–209 amu
Abundance Sensitivity0.3% for 159Tb
Instrument Response400,000 counts/pg 159Tb
Detection Limit500 antibodies/cell
Dynamic Range4.5 orders of magnitude
CalibrationAutomatic
Operating SystemWindows® 7 Pro 64-bit
Data Storage720GB RAID (mirrored)
Sample IntroductionDual loop, syringe
Peak Throughput1,000 events/sec
Flow Rate45 μL/min
Replicate Sample CV (normalized)<3%
Width97 cm (38 in)
Height132 cm (52 in)
Depth79 cm (31 in)
Weight285 kg (628 lb)


Chiller
Specification

Width38 cm (15 in)
Height64 cm (25 in)
Depth67 cm (27 in)
Weight81 kg (178 lb)


Workstation
Specification

Operating SystemWindows 7 Pro 64-bit
CPUIntel® Core™ i7 3930K
RAM8 GB 2,400 MHz DDR3
Hard Drives1.44 TB (6 x 240 GB SSD Corsair®
Force Series™ GS SATA3) in RAID 10 configuration
Data Storage720 GB RAID (mirrored)
VideoNVIDIA® NVS™ 310 x16 512 MB
Power Supply650 W Antec® TruePower®
Serial Adapter Board8 Port Native PCI Express RS232
MonitorViewSonic® 24 in LED
Keyboard/MouseLogitech® MK120 Wired
DVD/Blu-rayLG® SATA
MotherboardASUS® P9X79 WS PRO
EnclosureAntec Titan 650
Data File Sizeimd    0.3 MB/sec/channel
fcs     12.4 bytes/event/channel
txt      2.4 bytes/event/channel
Width20 cm (8 in)
Height46 cm (18 in)
Depth58 cm (23 in)
Weight35 kg (77 lb)



Autosampler (optional)
Specification

Sample Volume50–900 μL
Sample Format3 x 96-well plate, up to 2 mL deep well
Sample ResuspensionProbe pipet motion
Reagent ReservoirsCarrier (DIW)       250 mL
Wash Solution    250 mL
Rinse (DIW)         250 mL
Peak Throughput1,000 events/sec
Flow Rate45 μL/min
Replicate Sample CV (normalized)<3%
Width39 cm (16 in)
Height24 cm (10 in)
Depth36 cm (14 in)
Weight20 kg (44 lb)


Site Requirements

Argon GasSourceLiquid or compressed gas
Pressure522 ±7 kPa (80 ±1 psi)
Flow Rate20 L/m
Purity≥99.996%
Oxygen<5 ppm
Hydrogen<1 ppm
Nitrogen<20 ppm
Water<4 ppm
ExhaustFlow Rate280 L/s (600 cfm)
Heat Dissipation3,000 W (10,090 BTU/hr)
ElectricalCyTOF 2Two dedicated 30 A single-phase 220–240 V AC,50–60 Hz
Power Consumption2 x 4,500 VA
ChillerPowered through instrument
Power Consumption2,300 VA (via instrument)
Workstation6 A, 100–240 V AC, 50–60Hz
Power Consumption1,050 VA
Monitor6 A, 110–230 V AC, 50–60 Hz
Power Consumption100 VA
Autosampler4.2 A, 100–240 V AC, 50–60 Hz
Power Consumption100 VA


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