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Purpose –
A study was conducted to compare sample throughput and detection limits between conventional reverse phase HPLC/MS and UHPLC/MS using a novel “field free” APCI source. The study was performed on a 16 component mixture of PAHs.
Methods –
A recently introduced PerkinElmer “field free” APCI source was interfaced to a PerkinElmer Flexar TM FX-10 UHPLC system coupled to a Flexar SQ 300 MS detector.
Results –
Going from a 5 µm to a 1.9 µm particle size improved the detection limits by an average factor of 6.5 for the 16 component PAH
mixture and decreased the total run time by a factor of 3. The detection limits varied from 1.5 ng to 6.0 pg.