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Label-free drug uptake analysis of whole cells by MALDI-TOF MS
发布时间：
2021-02-22 14:33
来源：
布鲁克（北京）科技有限公司-质谱仪器
| 领域： | 其他生命科学,其他,肿瘤/癌症 | ||
| 样品： | transport protein OATP2B1 | 项目： | 质谱 |
| 参考： | \ | ||
| 方案文件名 | 下载 |
|---|---|
Label-free drug uptake analysis of whole cells by MALDI-TOF MS |
下载此篇方案 |
In this application note, we demonstrate a MALDI MS cellular drug uptake and inhibition assay [1]. It was conducted in HEK293 cells overexpressing the transport protein OATP2B1.
Estrone-3-Sulfate (E3S) is a substrate for this transporter and its uptake was investigated in a pilot screen with 294 drugs with potential OATP2B1 uptake inhibiting properties. The use of the rapifleX mass spectrometer combined with MPP software led to a measurement time of ~5 min per 384 well target plate enabling MALDI MS-based screening and determination of precise IC50 values in concentration-response experiments.
