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工商注册信息已核实!领域: | 细胞生物学,分子生物学,蛋白/抗体/蛋白质组,多组学/蛋白质组/代谢组/脂质组,生物制药/仿制药 | ||
样品: | Subtilisin | 项目: | Stability Analysis |
参考: | - |
方案文件名 | 下载 |
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HDX-MS Conformational Stability Analysis of Subtilisin with the maXis II ETD |
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Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) is a versatile technique used to study protein conformational stability, protein-protein and protein-ligand interaction.
Abstract
Here, the conformational stability of a subtilisin serine protease from Bacillus lentus is studied at pH 10.8, which is an applicationrelevant condition for enzymes for laundry applications. Importantly, no structure has yet been produced for these conditions, but with HDX-MS we were able to quickly probe the dynamic structure extensively. The analysis was performed on an automated setup including a HDX compatible liquid handler with LC and a maXis II UHR-Q-TOF with ETD providing high flexibility, stability and reproducibility.