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布鲁克timsTOF fleX组学和成像质谱系统

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参考报价: 面议 型号: timsTOF fleX™
品牌: 布鲁克 产地: 德国
关注度: 544 信息完整度:
样本: 暂无样本 典型用户: 暂无
类型Q-TOF/IT-TOF功能串联
产地属性欧洲

MALDI技术引导空间定位组学SpatialOMx ,架起组学和疾病的桥梁


布鲁克于ASMS2019上推出高度创新的新型质谱产品timsTOF fleX™质谱仪,包括适配于ESI timsTOF Pro™质谱的软件可切换的MALDI源。这种新型ESI/MALDI组合功能,可在单台质谱上实现空间定位组学SpatialOMx™。timsTOF fleX配备布鲁克独有的10 kHz SmartBeam™ 3D激光源,具有真正的像素保真度,可在高空间分辨率下进行快速、无标记的MALDI成像,同时完全保持timsTOF Pro在ESI模式下无与伦比的4D蛋白质组学和表型组学的灵敏度。

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利用这种独特的SpatialOMx方法,研究人员可以深入洞悉MALDI成像组织中的空间分子分布,从而指导4D组学分子表达研究,例如蛋白质、低水平癌抗原肽、脂质、聚糖、代谢物或那些用传统染色或标记技术无法观察到的分子。在MALDI指导下的SpatialOMx方法中,使用者可用随后由ESI-TIMS/PASEF的dda(数据依赖采集),或者dia(非数据依赖采集)4D蛋白质组学或4D脂质组学/代谢组学的数据,来开展细胞亚群的特异性靶向研究。两者现在都可以在同一台强大的仪器上进行,即timsTOF fleX,具有推进单细胞生物学研究所需的超高灵敏度,成为用RNA测序(RNA-seq)进行单细胞转录组学研究的完美补充。

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MALDI Guided SpatialOMx on the timsTOF fleX

肿瘤的分子描述和定义

癌症的微环境包含正常细胞、肿瘤细胞、结缔组织、血管和炎症等区域以及在不同时间点的状态变化。每个区域都有各自独特的化合物分子特征。研究者对疾病状态的分析在很大程度上依赖于对组织病理学的解释和建立描绘生物作用的分子谱图,以此弥补传统组学对疾病了解的不足。

SpatialOMx - 发现生物标志物的关键

癌细胞和其他疾病状态具有显著的遗传和表观遗传修饰特征,影响基因组表达水平。不管是观察蛋白质组、脂质组还是代谢组,这些分子的空间分布包含了解释样品的重要信息。如果某些化合物在空间上高度集中,或者分子在特定结构上共同分布,如果只对样品作均质化分析,就会丢失这一重要信息。因为在组织水平的通信网络是癌症生长不可或缺的一部分,没有空间信息来关联分子和亚细胞群之间的关系,基于组学的生物标记物发现就会受到限制。 

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timsTOF fleX对大鼠脑脂质的分子成像。在20μm空间分辨率下,在5小时内采集了312095个扫描谱图。

上图下方显示了SCiLS  Lab对该数据组处理产生的聚类成分图。谱图被汇集对应于不同大脑区域的相应位置。


为多组学分析引入空间维度

timsTOF fleX是一种多功能仪器,用于探索样品中的分子组成和变化。timsTOF fleX建立在Bruker领先的timsTOF Pro质谱平台上,具有多组学所要求的高速度和高灵敏度的ESI质谱分析功能,并结合了高空间分辨率的MALDI源和平台机械,解析生物分子分布和给组学分析增加空间维度。将蛋白质组学分析转化为空间蛋白质组学、脂质组学转化为空间脂质组学和代谢组学转化为空间代谢组学。

 

创建你自己的整合图谱库

复杂分子网络图的绘制揭示了细胞内网络中相互作用分子的复杂系统。使用SpatialOMx建立的可以解释通信机制的整合图谱库意味着了解更多有关细胞相互通信的方式及引起的变化:

  • 细胞如何与本地微环境互动,形成增长和调控过程;

  • 特定细胞分子网络如何相互作用,执行特定生物功能;

  • 分子网络如何影响表观遗传学修饰。  

timsTOF fleX 在空间代谢组学、空间脂质组学或空间蛋白质组学之间灵活切换

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Combining the world’s fastest proteomics instrument with over 25 years of MALDI experience

Match PASEF powered LC-MS/MS identification with label-free spatial localization to decode the molecular make up of your sample

Built on the standard for shotgun proteomics, the timsTOF fleX combines best in class 4D X-Omics with Bruker’s cutting edge MALDI Imaging technology, including SmartBeam 3D laser optics for fast measurement all in one platform. A dual source instrument ideal for SpatialOMx, timsTOF fleX conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform. No single instrument has before provided access to both essential capabilities for the most advanced OMICS researchers. 

From 4D OMICS to molecular imaging without stopping, changing, modifying or compromises

SpatialOMx demands PASEF enabled LC-MS/MS for deep 4D OMICS combined with the ability to create molecular images of tissue sections with MALDI Imaging. To do this under one platform, Bruker engineers started with the award winning TIMS dual-stage ion funnel and incorporated target stage and laser elements from the rapifleX axial TOF instrument.  During operation, software activation of the SmartBeam 3D laser is the only change in the source region.  No complicated changeover means making zero compromises in productivity and the ability to move effortlessly from world class OMICS identification and quantification workflows to creating high definition molecular maps of tissue sections to see what matters most. 

Since ions produced by MALDI and ESI travel the same path to the detector from the source, MALDI workflows can take advantage of the most advanced features found in the timsTOF Pro, including TIMS ion mobility separation based on the collisional cross section of detected molecules and high-speed high-sensitivity PASEF fragmentation. Tuning and calibration can be performed in ESI mode and used for the MALDI experiment for additional ease of optimization.

Direct molecular profiling from tissue with MALDI Imaging

MALDI Imaging is a label-free tool for examining the distribution of molecules directly from thin tissue sections. Applicable for examining a wide range of analytes, including metabolites, lipids or glycans, and seamlessly compatible with microscopy workflows, MALDI Imaging can identify regionally specific compound distributions for SpatialOMx analyses. Use Bruker’s IntelliSlides for automated MALDI Imaging and SpatialOMx workflows on the timsTOF flex.

Zoom mode to see what really matters

Besides the ability to generate high definition “True Pixel” MALDI images, the SmartBeam 3D laser source provides rock solid laser guided targeting down to 10 µM with Bruker’s new zoom mode technology. Users now have the flexibility to adjust their laser spot size and spacing to accommodate a wider range of granularity depending on biological features of interest.


Combining X-Omics performance with high MALDI sensitivity

timsTOF fleX uniquely enables SpatialOMx

Whether it’s proteomics, lipidomics, glycomics, or metabolomics, the timsTOF fleX is the ideal platform for deep SpatialOMx analyses. Use patented SmartBeam™ 3D technology to conduct fast, label-free MALDI Imaging for mapping the distribution of molecules in your sample and identify regions of interest for more specific analyses. Apply PASEF powered LC-MS/MS for the highest level of identifications and for greatest confidence in your results.

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High speed and high sensitivity for biomarker discovery

Combining the power of trapped ion mobility spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF), the timsTOF fleX can deliver quantitative information from smaller amounts of highly complex mixtures. Using less than 200 ng sample load and a 90 min gradient length, more than 5400 protein groups can be identified from a typical human cell line lysate. The high sensitivity reduces sample preparation costs and decreases time lost to maintenance.

MALDI Imaging for investigating compound distributions

With existing protocols for lipidomics, metabolomics or glycomics, MALDI Imaging spectra can be used as a molecular fingerprint for specific regions, or to investigate how compounds are localized across an entire sample for biomarker discovery. This can also be used for investigation of temporal changes, quantification, and correlation of compounds to tissue states. The timsTOF fleX allows for fast high mass resolution MALDI imaging – with the SmartBeam 3D and specialized MALDI source and stage, a high spatial resolution measurement of lipids from a rat brain section consisting of 370,000 pixels takes less than 5 hours.

MALDI guided SpatialOMx for laser precise efficiency

Traditional workflows that sample small tissue sections (<100 µM) provide deep molecular details for a variety of chemical classes including RNA/DNA, proteins, glycans, lipids and metabolites. However, processing times are long and tissue excise locations are determined with optical images that lack molecular signal information. SpatialOMx on the timsTOF fleX rapidly provides a high definition, label-free overview that merges chemical information with vital morphological context. Choose your regions of interest manually or use SCiLS enabled automated segmentation to know exactly where your extraction will have the highest impact.

Introducing an automated molecular annotation workflow for SpatialOMx

Using Bruker’s industry leading software, it is now possible to annotate target molecules directly from tissue. Simply load your data into SCiLS Lab, define regions of interest, and export the peak lists to MetaboScape. Annotate your peaks using databases or compound lists from LC-MS/MS analyses, then review and export your annotations back to SCiLS for visualization. From SCiLS you can then visualize results with pathways and familiar nomenclature rather than mass labels to reduce the time from data to results. 

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布鲁克道尔顿执行副总裁Rohan Thakur博士补充说:“大多数组织蛋白质组学研究融合了来自不同亚群的细胞,其不期望的平均效应掩盖了许多重要的生物学和病理生物学信息。在timsTOF fleX上,MALDI引导的SpatialOMx方法能够在空间上对指定的组织区域进行分析,从而允许随后的4D蛋白质组学选择性地对细胞类型的亚群进行靶向分析。功能强大、具有超高灵敏度的timsTOF fleX,在同一仪器上还提供nanoLC-TIMS-MS/MS,从而弥合了分子组织成像和体液分析间的鸿沟。这种独特的组合将使timsTOF fleX成为宝贵的研究工具,用于空间分辨的4D蛋白质组学和表型组学,并推动单细胞生物学、药物蛋白质组学和大细胞数量或患者队列的转化4D交叉组学的工作流程。”

Helmholtz药物研究所(HIPS)助理教授Daniel Krug博士评论说:“我们在药物研究和空间代谢组学领域使用质谱技术;尤其是分析新型天然产物的粘细菌次级代谢组。我们实验室多年来一直使用布鲁克的仪器和软件,特别是SCiLS Lab和MetaboScape;新分子成像工作流程集成了这两种软件解决方案,极大地简化并加速了整个MALDI成像流程。该流程将离子图像转换为分子图像,并提高了注释分子式的置信度。”


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注:该仪器未取得中华人民共和国医疗器械注册证,不可用于临床诊断或治疗等相关用途

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