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Rapid Coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip based Mass Spectrometry for the Identification of Proteins in Nucleoprotein Interactions
发布时间：
2016-10-07 17:44
来源：
HORIBA科学仪器事业部
| 领域： | 分子生物学 | ||
| 样品： | 蛋白质 | 项目： | 生物大分子相互作用，质谱分析 |
| 方案文件名 | 下载 |
|---|---|
Rapid Coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip based Mass Spectrometry for the Identification of Proteins in Nucleoprotein Interactions |
下载此篇方案 |
Results demonstrate that monolayer surfaces permit accurate SPR analysis of macromolecular interactions and that the SPRi-PLEX configuration is well suited to a simple recovery protocol that provides rapid sensitive mass spectrometry analysis. Clearly at these levels of surface saturation more than 100 spots could be rapidly visualized and the material recovered for analysis by SELDI even with the manual approach used here.
