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使用源柱一体PicoChip肌钙蛋白的常规定量方法
发布时间：
2015-05-05 17:09
来源：
New Objective China
| 样品： | 蛋白质 | 项目： | 蛋白质质谱分析 |
下载地址: 使用源柱一体PicoChip肌钙蛋白的常规定量方法
Liquid chromatography/tandem mass spectrometry offers great potential for quantitative proteomics, but optimal workflows and system configurations still needs to be established. In this study we developed, validated and compared three procedures for absolute quantitation of cardiac troponin T (cTnT) using microflow LC/MS or chip-‐based nanospray source with direct injection or preconcentration nanoflow LC/MS. Troponin T content was evaluated in hearts of C57BL/6 and ApoE/LDLR double knock-‐out mice.