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用全自动芯片电喷雾离子化和高容量离子阱质谱分析神经节苷脂
发布时间：
2014-03-12 16:53
来源：华质泰科生物技术（北京）有限公司
| 资料类型： |
| 资料文件名 | 下载 |
|---|---|
Zamfir et al Anal Biochem 2008 - Ganglioside Analysis.pdf |
下载此篇资料 |
Anal Biochem. 2008 Jul 1;378(1):43-52. doi: 10.1016/j.ab.2008.03.039. Epub 2008 Mar 28.
Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis.
Abstract
NanoMate robot was coupled to a high-capacity ion trap (HCT) mass spectrometer to create a system merging automatic chip-based electrospray ionization (ESI) infusion, ultrafast ion detection, and multistage sequencing at superior sensitivity. The interface between the NanoMate and HCT mass spectrometer consists of an in-laboratory constructed mounting device that allows adjustment of the robot position with respect to the mass spectrometer inlet.
Automated chip-based nanoelectrospray
Fully automated chip-based nanoelectrospray was performed on a NanoMate BiVersa robot incorporating ESI 400 chip technology (Advion Biosciences, Ithaca, NY, USA) controlled and manipulated by ChipSoft 7.1.1 software operating under a Windows system. The robot was coupled to the HCT Ultra mass spectrometer via an in-laboratory made mounting system.
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