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集成运用鸟枪法脂质组学和用HPLC分离脂质法,建立脂质分析新平台

发布时间: 2014-03-11 16:51 来源:华质泰科生物技术(北京)有限公司
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EjsingFC-ASMS2010_cse_230x120_3.pdf

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Poster

Integrating shotgun lipidomics and HPLC separation of lipids into a new platform for lipid analysis  

【Summary 】
  • Development of a lipidomics platform based on direct infusion and LC MS analysis
  • LC MS analysis by normal phase chromatography and nanoflow split to a chip-based infusion device 
  • Implementation of a fraction collection strategy for indepth profiling of lipid species by direct infusion analysis 
  • This platform is a powerful tool for arraying the lipidome of poorly characterised organisms 
Ion source/Fraction collector:
NanoMate Triversa Sample analysis was performed by LC-coupling/fraction collection mode using a post-column split where 50 nl/min was directed to the ESI-chip and 49.5 µl/min was use for parallel fraction collection.  
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